Investigation of Purification Technology for Effective Constituents from  by Different Types of Macroporous Resin

SHENG Hua-gang; ZHU Li-qiao

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Investigation of Purification Technology for Effective Constituents from by Different Types of Macroporous Resin

更新时间：2024-01-04

- Investigation of Purification Technology for Effective Constituents from by Different Types of Macroporous Resin
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 24, Pages: 41-44(2012)
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- Published：2012
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SHENG Hua-gang, ZHU Li-qiao. Investigation of Purification Technology for Effective Constituents from by Different Types of Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(24): 41-44.
DOI：

SHENG Hua-gang, ZHU Li-qiao. Investigation of Purification Technology for Effective Constituents from by Different Types of Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(24): 41-44. DOI：

摘要

目的: 考察不同型号大孔树脂对金荞麦中有效成分的纯化效果。方法: 以表儿茶素和金E的含量、浸膏率为指标

结合HPLC指纹图谱

选择最佳型号大孔树脂。结果: AB-8型大孔树脂对表儿茶素洗脱率为89.78%

对金E洗脱率为93.04%。HPLC指纹图谱共有峰面积比较表明AB-8型大孔树脂最适合用于金荞麦有效成分的纯化。结论: 采用AB-8型大孔树脂纯化金荞麦中有效成分是可行的

且操作简单、稳定。

Abstract

Objective:To investigate purification effect of different types of macroporous resin on effective constituents from Fagopyrum dibotrys. Method: With the content of epicatechin and mixture of proanthocyanidins tannic condensation

extract rate as indexes

optimum resin was selected by combining with HPLC fingerprint. Result: Elution rate of epicatechin by AB-8 resin was 89.78%

elution rate of mixture of proanthocyanidins tannic condensation by AB-8 resin was 93.04%.Comparison of the common peak area of HPLC fingerprint showed that AB-8 resin was the most suitable one to purify effective constituents from F. dibotrys. Conclusion: This process of applying AB-8 resin to purity effective constituents from F. dibotrys was feasible

simple and stable and could be extended to large-scale production applications.

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Related Author

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CAI Xiong

LAI Xiao-ping

ZHU Chen-chen

LIU Liang

PAN Jun1

YU Qing-hua1

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Related Institution

School of Pharmacy,Shandong University of Traditional Chinese Medicine,Ji’nan

Zhejiang Chinese Medical University

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

State Key Laboratory of Southwestern Traditional Chinese Medicine（TCM） Resources， School of Pharmacy，Chengdu University of TCM

Sichuan Neo-green Pharmaceutical Technology Development Co. Ltd.，Pengzhou

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