Optimization of Extraction and Precipitation Technology for Liuyueqing Polysaccharide Capsule by Orthogonal Design

GE Wen-yi; HUANG Jian-chun; CHEN Zhao-ni; JIAO Yang; ZHANG Shi-jun; HUANG Ren-bin

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Optimization of Extraction and Precipitation Technology for Liuyueqing Polysaccharide Capsule by Orthogonal Design

更新时间：2024-01-04

- Optimization of Extraction and Precipitation Technology for Liuyueqing Polysaccharide Capsule by Orthogonal Design
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 21, Pages: 42-45(2012)
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- Published：2012
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GE Wen-yi, HUANG Jian-chun, CHEN Zhao-ni, et al. Optimization of Extraction and Precipitation Technology for Liuyueqing Polysaccharide Capsule by Orthogonal Design[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(21): 42-45.
DOI：

GE Wen-yi, HUANG Jian-chun, CHEN Zhao-ni, et al. Optimization of Extraction and Precipitation Technology for Liuyueqing Polysaccharide Capsule by Orthogonal Design[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(21): 42-45. DOI：

摘要

目的: 优选六月青多糖胶囊提取、醇沉工艺条件。方法: 以多糖含量为指标

采用正交试验考察料液比、煎煮次数及煎煮时间对六月青多糖胶囊提取工艺的影响;以多糖含量及多糖收率为综合评价指标

选取醇沉时间、醇沉次数、加醇量及醇沉浓度为考察因素

通过正交试验优选醇沉工艺;采用苯酚-浓硫酸法测定总多糖含量。结果: 六月青多糖胶囊最佳提取工艺为加10倍量水煎煮3次

每次煎煮2 h。最佳醇沉条件为水提液过滤后浓缩至0.5 g·mL-1

加7倍量95%乙醇沉淀12 h

沉淀1次。醇沉后多糖纯度达71.05%

收率6.83%。结论: 优选的提取及醇沉工艺条件稳定合理

可为六月青多糖胶囊的生产提供实验依据。

Abstract

Objective: To optimize extraction and precipitation technology conditions of Liuyueqing polysaccharide capsule. Method: With the content of polysaccharide as index

orthogonal test was adopted to investigate influence of solid-liquid ratio

decoction times and decoction time on extraction technology of Liuyueqing polysaccharide capsule;With the content and yield of polysaccharide as comprehensive evaluation index

alcohol precipitation time and times

the amount of ethanol and alcohol precipitation concentration were chosen as factors

alcohol precipitation technology was optimized by orthogonal test.The content of total polysaccharides was determined by phenol-concentrated sulfuric acid method. Result: Optimal extraction technology of Liuyueqing polysaccharide capsule was as follows:decocted three times with 10-fold water

2 hours each time.Optimum alcohol precipitation conditions were:filtered extraction liquid

mixed and concentrated to 0.5 g ·mL-1

and then precipitated one time with 7-fold 95% ethanol for 12 hours.Under these conditions

purity of polysaccharide was up to 71.05%

yield of it was 6.83%. Conclusion: This optimized extraction and precipitation procedure was proved to be stable and reliable

it could provide experimental basis for Liuyueqing polysaccharide capsule.

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Related Institution

Guiyang College of Traditional Chinese Midicine

Second Affiliated Hospital of Guiyang College of Traditional Chinese Midicine

College of Pharmacy, Chengdu University of TCM

Key Laboratory of Active Ingredients in Traditional Chinese Medicine(TCM) Co-constructed by Jilin Province and Ministry of Education, Changchun University of Chinese Medicine

Research and Development Centre of New Drugs,Guangxi University of Chinese Medicine

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