Objective:To establish an RP-HPLC method for simultaneous determination of aconitine

hypaconitine and mesaconitine in Sini decoction. Method: Hypersil C18 was used as chromatographic separation column

and methanol-0.2% triethylamine (pH adjusted to 5.3 with CH3COOH) was used as the mobile phase. The detection wavelength was set at 230nm. The flow rate was 1.0 mL·min-1 and the column temperature was set at 25 ℃. Result: Aconitine

hypaconitine and mesaconitine were linear in the range of 0.875-28 mg·L-1 (r=0.999 8)

1.125-36 mg·L-1 (r=0.999 9) and 0.812-26 mg·L-1 (r=0.999 8)

respectively. Their average recoveries were 94.9% for aconitine

100.2% for hypaconitine and 99.9% for mesaconitine

respectively. Conclusion: The established method is convenient and can be helpful for the quality control of Sini decoction.