Liquid Chromatography-Mass Spectrography Fingerprinting Analysis of Flos Gentianae Stramienae

HU Yi-chen; JI Lang; LIAO Qing; CAO Ji-liang; WAN Li

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Liquid Chromatography-Mass Spectrography Fingerprinting Analysis of Flos Gentianae Stramienae

更新时间：2024-01-04

- Liquid Chromatography-Mass Spectrography Fingerprinting Analysis of Flos Gentianae Stramienae
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 2, Pages: 67-70(2013)
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- Published：2013
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HU Yi-chen, JI Lang, LIAO Qing, et al. Liquid Chromatography-Mass Spectrography Fingerprinting Analysis of Flos Gentianae Stramienae[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(2): 67-70.
DOI：

HU Yi-chen, JI Lang, LIAO Qing, et al. Liquid Chromatography-Mass Spectrography Fingerprinting Analysis of Flos Gentianae Stramienae[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(2): 67-70. DOI：

摘要

目的: 建立秦艽花的液质联用指纹图谱检测方法

为最终实现秦艽花真伪品的鉴别提供参考。方法: 以Agilent ZORBAX ECLIPSE XDB-C18(2.1 mm×50 mm

1.8 μm) 为分析用色谱柱

0.1%甲酸水溶液-乙腈为流动相进行梯度洗脱

在正离子模式下

分析11批秦艽花药材

并利用中药色谱指纹图谱相似度评价系统进行了相似度计算。结果: 建立了秦艽花液质联用指纹图谱的共有模式

标定了指纹图谱中的23个共有峰

11批药材的相似度均>0.90

并用HPLC-ESI-Q-TOF-MS技术对7个共有峰进行了指认。结论: 方法精密度、稳定性和重复性良好

为提高秦艽花的质量标准提供了依据。

Abstract

Objective: To establish the fingerprint chromatograms of Flos Gentianae Stramienae by the means of liquid chromatography-mass spectrography (LC-MS). Method: The analysis was performed on Agilent ZORBAX ECLIPSE XDB-C18 column (2.1 mm×50 mm

1.8 μm) with the mobile phase consisting of 0.1% formic acid-water-acetonitrile in a gradient mode. The chromatograms were recorded by using ESI-MS in positive ion model. Totally

11 batches of samples were analyzed and their similarities were calculated using fingerprint similarity evaluation software. Result: The LC-MS fingerprint common mode of Flos Gentianae Stramienae was obtained. A total of 23 characteristic peaks were selected as the common fingerprint peaks. The similarities of 11 samples were greater than 0.90 as compared to the common mode and seven peaks were identified by using HPLC-ESI-Q-TOF-MS. Conclusion: The reported method is precise

reproducible and reliable. The establishment of LC-MS fingerprint chromatograms can be helpful for the quality control of Flos Gentianae Stramienae.

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Related Institution

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

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Modern Research Center for Traditional Chinese Medicine，Shanxi University

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