Determination of Acteoside and Luteolin in by HPLC
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Determination of Acteoside and Luteolin in by HPLC
Chinese Journal of Experimental Traditional Medical FormulaeVol. 19, Issue 1, Pages: 81-84(2013)
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Published:2013
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CAI Jin-ping, DONG Lin, SHENG Lin, et al. Determination of Acteoside and Luteolin in by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(1): 81-84.
DOI:
CAI Jin-ping, DONG Lin, SHENG Lin, et al. Determination of Acteoside and Luteolin in by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(1): 81-84.DOI:
Determination of Acteoside and Luteolin in by HPLC
Objective:To establish an HPLC method for determination of acteoside and luteolin in Callicarpa nudiflora. Method: HPLC method was applied on Phenomenex Gemini C18 column (4.6 mm×250 mm
5 μm) at 30℃ under the condition of gradient elution with mobile A (methanol) and mobile B (0.3% phosphate). The flow rate was 1.0 mL·min-1
the detection wavelength was set at 350 nm. Result: Two components had a good liner relationship in concentration of 29.6-740.0
1.0-25.0 μg (r=0.999 9). The average recoveries were 99.08%
99.93% and the RSD was 1.53%
1.65% respectively. Conclusion: The method is accurate and reproducible for determination of acteoside and luteolin in C. nudiflora.