Simultaneous Determination of Puerarin, Liquiritin, Baicalin and Berberine Hydrochloride in Gegenqinlian Micropills by RP-HPLC
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Simultaneous Determination of Puerarin, Liquiritin, Baicalin and Berberine Hydrochloride in Gegenqinlian Micropills by RP-HPLC
Chinese Journal of Experimental Traditional Medical FormulaeVol. 19, Issue 2, Pages: 64-67(2013)
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Published:2013
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SONG Ya-fang, SU Chun-mei, YANG Hong, et al. Simultaneous Determination of Puerarin, Liquiritin, Baicalin and Berberine Hydrochloride in Gegenqinlian Micropills by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(2): 64-67.
DOI:
SONG Ya-fang, SU Chun-mei, YANG Hong, et al. Simultaneous Determination of Puerarin, Liquiritin, Baicalin and Berberine Hydrochloride in Gegenqinlian Micropills by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(2): 64-67.DOI:
Simultaneous Determination of Puerarin, Liquiritin, Baicalin and Berberine Hydrochloride in Gegenqinlian Micropills by RP-HPLC
Objective: To establish a method for simultaneous determination of puerarin
liquiritin
baicalin and berberine hydrochloride in Gegenqinlian Micropills. Method: RP-HPLC was applied on a Kromasil-C18 column (4.6 mm×250 mm
5 μm) with methanol-phosphate buffer as mobile phase by gradient elution. The flow rate was 1.0 mL· min-1
and the detection wavelength was set at 275 nm. Result: The methodology of 4 components met the requirements with no interference of negatives. Conclusion: The method established is easy
accurate with high specificity
which can provide the base for the quality control of Gegenqinlian Micropills.
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