Determination of Specnuezhenide in Nuzhen Granules by HPLC
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Determination of Specnuezhenide in Nuzhen Granules by HPLC
Chinese Journal of Experimental Traditional Medical FormulaeVol. 19, Issue 4, Pages: 153-155(2013)
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Published:2013
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SHANG Gui-chun, YUAN Da-peng, LIU Hai-bin. Determination of Specnuezhenide in Nuzhen Granules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(4): 153-155.
DOI:
SHANG Gui-chun, YUAN Da-peng, LIU Hai-bin. Determination of Specnuezhenide in Nuzhen Granules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(4): 153-155.DOI:
Determination of Specnuezhenide in Nuzhen Granules by HPLC
Objective: To establish a method for determination of specnuezhenide in Nuzhen granules. Method: Samples were analyzed on a Waters-C18 column (4.6 mm×250 mm
5 μm) using methanol-water (40: 60) as mobile phase. Flow rate was 1.0 mL·min-1 and examination wavelength was set at 224 nm. Result: Specnuezhenide showed a good linearity in the range of 0.306 25-0.918 75 μg
regression equation was Y=4×10-7X-1.51×10-2(r=0.999) and the average recovery was 98.00% (RSD 1.48%
n=6). Conclusion: The established methods are simple
accurate
reliable and can be used for the quality control of Nuzhen granules.