Objective:To prepare Saussurea involucrata microemulsion

investigate relationship between retention volume and time of of S. involucrata microemulsion in abdominal skin of mice

investigate its pharmacokinetics of local transdermal drug delivery

and investigate its skin absorption effect. Method: Characteristics of prepared S. involucrata microemulsion were determined including polydispersity index

size distribution and Zeta potential and other physical and chemical properties.With chlorogenic acid and rutin as indexes

biological retention volume of chlorogenic acid and rutin in administration skin with different time was determined by HPLC

and pharmacokinetic parameters were fitted by DAS software. Result: Characteristics of prepared S. involucrata microemulsion including mean particle size

polydispersity index and Zeta potential were 54.51 nm

0.281 and-0.687 mV.Pharmacokinetic process of microemulsion in rats' abdominal skin was fitted with two compartment model. Conclusion: Active ingredients of S. involucrata microemulsion could quickly permeate into skin

and accumulate long time in local skin.