Effect of -acetylcysteine on Oxidative Stress in Acute Kidney Injury Induced by Cisplatin

LUO Jing-hui; YANG Ying-bao

您当前的位置：

首页 >

文章列表页 >

Effect of -acetylcysteine on Oxidative Stress in Acute Kidney Injury Induced by Cisplatin

更新时间：2024-01-04

- Effect of -acetylcysteine on Oxidative Stress in Acute Kidney Injury Induced by Cisplatin
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 19, Pages: 170-175(2012)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2012
- 稿件说明：
移动端阅览
LUO Jing-hui, YANG Ying-bao. Effect of -acetylcysteine on Oxidative Stress in Acute Kidney Injury Induced by Cisplatin[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(19): 170-175.
DOI：

LUO Jing-hui, YANG Ying-bao. Effect of -acetylcysteine on Oxidative Stress in Acute Kidney Injury Induced by Cisplatin[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(19): 170-175. DOI：

摘要

目的:研究顺铂(cisplatin

CDDP)诱导大鼠急性肾损伤(acutekidneyinjury

AKI)后肾脏组织氧化应激水平及N-乙酰半胱氨酸(N-acetylcysteine

NAC)的干预作用。方法:静脉注射CDDP制备大鼠AKI模型。大鼠随机分为空白对照组、AKI模型对照组、NAC低、中、高剂量组(50

100

200mg·kg-1)、维生素C(VitC)对照组(100mg·kg-1)。大鼠预先连续给药3d后

给予CDDP

再继续给药5d。测定大鼠体质量、肾质量、肾脏指数、尿蛋白排泄率。试剂盒测定肾脏组织乳酸脱氢酶(LDH)、丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、还原型谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-Px)、15-F2t-异前列烷(15-F2t-Isop)水平。免疫组化法观察8-OHdG阳性细胞。Westernblot测定Cu-Zn-SOD蛋白表达。结果:与空白对照组相比

CDDP诱导AKI模型组体质量、肾质量、肾脏指数下降

尿蛋白排泄率由(0.43±0.09)g显著增加为(2.24±0.41)g

LDH

MDA

15-F2t-Isop

8-OHdG阳性细胞显著增加

CAT

SOD

GSH

GSH-Px和Cu-Zn-SOD表达显著下降(P<0.01)。与AKI模型组相比

NAC组尿蛋白排泄率降低为(0.56±0.19)g

具有显著差异。同时

AKI模型组肾脏指数

LDH

MDA

SOD

CAT

15-F2t-Isop

GSH

GSH-Px水平和8-OHdG阳性细胞数分别为(2.71±0.55)×10-2

(6.92±0.51)KU·mg-1

(68.6±9.2)nmol·mg-1

(61±15)U·mg-1

(5.12±1.04)U·mg-1

(63.2±9.62)ng·mg-1

(159±18)nmol·mg-1

(72±15)U·mg-1

(12.25±2.23)个

NAC组200mg·kg-1组分别为(2.96±0.32)×10-2

(4.62±0.26)KU·mg-1

(39.5±7.9)nmol·mg-1

(109±20)U·mg-1

(9.85±1.21)U·mg-1

(41.8±4.65)ng·mg-1

(225±16)nmol·mg-1

(128±22)U·mg-1

(3.26±0.96)个

两组相比较

具有非常显著差异(P<0.01)

Cu-Zn-SOD表达显著增加(P<0.01)。结论:NAC有效拮抗CDDP的肾损伤作用

可能与其增强肾脏组织抗氧化活性、降低CDDP诱导AKI时肾脏组织氧化应激水平有关。

Abstract

Objective:To investigate the effect of N-acetylcysteine (NAC) on acute injured kidney (AKI) induced by cisplatin (CDDP). Method:The rats were injected CDDP intravenously to make AKI model. The rats were randomly divided into 6 groups

including the normal control group

CDDP-induced AKI model group

NAC groups (50

100

200 mg·kg-1) and Vit C control group (100 mg·kg-1). NAC and Vit C were administered once a day three days before CDDP was given. And then NAC and Vit C were continuously given ip for five days. The body weight

kidney weight

kidney index

urinary protein excretion rate of rats were determined. The commercial kits were employed to test the concentrations of lactate dehydrogenase (LDH)

malondialdehyde (MDA)

superoxide dismutase (SOD)

catalase (CAT)

reduced glutathione (GSH)

glutathione peroxidase (GSH-Px)

15-F2t-isoprostane (15-F2t-Isop). Eight-OHdG positive cells were counted by immunohistochemical staining. The expression of Cu-Zn-SOD was measured by Western blot. Result:Compared with the normal control group

the weight of body and kidney

kidney index

the concentration of CAT

SOD

GSH

GSH-Px and Cu-Zn-SOD expression were significantly decreased

while urinary protein excretion rate and the concentration of LDH

MDA

15-F2t-Isop

8-OHdG positive cells were significantly increased (P<0.01) in the CDDP-induced kidney injury group. NAC and Vit C significantly reduced urinary protein excretion rate and the concentration of LDH

MDA

15-F2t-Isop

8-OHdG positive cells and increased kidney index

the concentration of CAT

SOD

GSH

GSH-Px and Cu-Zn-SOD expression compared with the AKI model group (P<0.01). Conclusion:NAC protect kidney from CDDP injury by suppressing the oxidative stress.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Pharmacokinetic Analysis of Ziyuglycoside Ⅰ in Normal and Acute Kidney Injury Rats

Mechanism of Fuzi Lizhongwan Improving Injury in Cisplatin-induced CIPN Mice by Regulating MAPK Signaling Pathway

Astragali Radix-Salviae Miltiorrhizae Radix et Rhizoma Ameliorates Myocardial Ischemia-reperfusion Injury in Rats via FUNDC1-dependent Mitophagy

Shengxiantang Alleviates Idiopathic Pulmonary Fibrosis in Rats by Regulating Oxidative Stress to Inhibit PANoptosis

Related Author

ZHANG Yunhui

LIU Yanli

XU Qiongming

SUN Shuding

ZHU Hongjin

ZHAO Di

FENG Suxiang

Zheng-yun ZUO

Related Institution

Henan University of Chinese Medicine

Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases Co-constructed by Henan province & Education Ministry of P.R. China

Soochow University

Formula-pattern Research Center of Jiangxi University of Chinese Medicine

College of Traditional Chinese Medicine， Jiangxi University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰