Determination of Total Aristolochic Acid in Different Extracts of

YU Yan; XIU Li-li; WEI Xin-zhi; SUN Ke-feng

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Determination of Total Aristolochic Acid in Different Extracts of

更新时间：2024-01-04

- Determination of Total Aristolochic Acid in Different Extracts of
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 24, Pages: 141-143(2012)
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- Published：2012
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YU Yan, XIU Li-li, WEI Xin-zhi, et al. Determination of Total Aristolochic Acid in Different Extracts of [J]. Chinese journal of experimental traditional medical formulae, 2012, 18(24): 141-143.
DOI：

YU Yan, XIU Li-li, WEI Xin-zhi, et al. Determination of Total Aristolochic Acid in Different Extracts of [J]. Chinese journal of experimental traditional medical formulae, 2012, 18(24): 141-143. DOI：

摘要

目的: 测定北细辛不同提取物中总马兜铃酸的含量

为其临床应用提供实验依据。方法: 以水、乙酸乙酯、30%乙醇、50%乙醇、70%乙醇、95%乙醇提取北细辛药材

以马兜铃酸A为对照

采用紫外分光光度法

检测波长为390 nm。结果: 马兜铃酸A含量在3.0~30.0 mg·L-1与吸收度呈良好线性关系

Y=19.844X+0.027 2(r=0.999 6)

平均回收率为98.51%

RSD 1.32%。北细辛不同提取物中总马兜铃酸的含量为1.34%~3.15%

以水提取物含量最低

95%乙醇提取物含量最高。结论: 方法测定结果准确可靠

重复性好

可用于北细辛提取物中总马兜铃酸的含量测定。

Abstract

Objective:To determine the content of total aristolochic acid in different extracts of Asarum heterotropoides

and provide the basis for clinical application of A. heterotropoides. Method: A. heterotropoides was extracted with water

ethyl acetate

30% ethanol

50% ethanol

70% ethanol and 95% ethanol as extracting solvent. The ultraviolet spectrophotometry was used with aristolochic acid A as the chemical reference. The UV detector wavelength was set at 390 nm. Result: The content of aristolochic acid A showed a linear relation with the spectrophotometric absorbance within the range of 3.0-30.0 mg·L-1

and the linear equation was Y=19.844X+0.027 2 (r=0.999 6). The average recovery was 98.51%

and RSD was 1.32%. The total aristolochic acid content varied from 1.34%-3.15%. It was the lowest in the water extract and highest in the 95% ethanolic extract. Conclusion: The method is reliable

accurate and suitable for determining the content of the total aristolochic acid in extracts of A. heterotropoides.

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