Objective: The everted gut scas in vitro was used to investigate intestinal absorption kinetics characteristics of paeoniflorin and ferulic acid from Yangxue Qingnao granule at different intestinal section. Method: In vitro everted gut model was applied. The absorption concentration of paeoniflorin and ferulic acid

was detected by HPLC

and the absorption parameter was calculated for describing the absorption characters. Result: The absorption of paeoniflorin and ferulic acid was linearity in different intestine

and the square of coefficient correlation exceed 0.9

which consistent with zero order rate process. The absorption of paeoniflorin and ferulic acid in ileum was quick. Conclusion: The absorption of paeoniflorin and ferulic acid in Yangxue Qingnao granule conforms to the zero order rate process at different sections of intestine.