Effects of Huoxiang Zhengqi Liquid on the Expression of AQP4 in Colon Mucosa in Rats with Dampness Retention Syndrome

XUE Xiao-qian; HUANG Xue-kuan; GAO Ning; LIU E-hu; REN Ling-yan

您当前的位置：

首页 >

文章列表页 >

Effects of Huoxiang Zhengqi Liquid on the Expression of AQP4 in Colon Mucosa in Rats with Dampness Retention Syndrome

更新时间：2024-01-04

- Effects of Huoxiang Zhengqi Liquid on the Expression of AQP4 in Colon Mucosa in Rats with Dampness Retention Syndrome
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 19, Pages: 165-169(2012)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2012
- 稿件说明：
移动端阅览
XUE Xiao-qian, HUANG Xue-kuan, GAO Ning, et al. Effects of Huoxiang Zhengqi Liquid on the Expression of AQP4 in Colon Mucosa in Rats with Dampness Retention Syndrome[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(19): 165-169.
DOI：

XUE Xiao-qian, HUANG Xue-kuan, GAO Ning, et al. Effects of Huoxiang Zhengqi Liquid on the Expression of AQP4 in Colon Mucosa in Rats with Dampness Retention Syndrome[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(19): 165-169. DOI：

摘要

目的:研究藿香正气液对湿阻证大鼠结肠黏膜水通道蛋白4(AQP4)表达的影响。方法:将24只SD大鼠随机分为正常组、模型组、藿香正气液组

每组8只。正常组常规喂养

其余各组采用改进的环境加疲劳法制造湿阻证模型

连续造模6d。造模成功后

正常组和模型组予生理盐水ig(20mL·kg-1)

治疗组予藿香正气液ig(20mL·kg-1)

1次/d

连续8d。分别采用免疫组化和Real-TimePCR的方法观察结肠黏膜AQP4的表达情况。结果:与正常组相比

模型组大鼠脾虚症状明显

胃黏膜有明显充血水肿

并有出血点及血块

大鼠结肠黏膜AQP4表达明显减少(P<0.05);而经藿香正气液治疗后

与模型组比较

AQP4表达量有所增加(P<0.05)。结论:藿香正气液可明显改善湿阻证大鼠脾虚腹泻等症状

其机制可能与提高结肠黏膜AQP4的表达量有关。

Abstract

Objective:To investigate the effects of Huoxiang Zhengqi liquid on the expression of AQP4 in colon mucosa in rats with dampness retention syndrome (DRS). Method:Twenty four SD rats were randomly divided into 3 groups:control group

DRS group and treatment groups (DRS+Huoxiang Zhengqi liquid)

8 rats in each group.DRS was induced in all rats

except the control group

by modified environmental and fatigued method for 6 days.After DRS model was copied

control and DRS groups were orally given with normal saline(NS

20 mL·kg-1). Rats in treatment groups were orally given with Huoxiang Zhengqi liquid(20 mL·kg-1) for 8 days.Then the expression of aquaporin 4(AQP4) on colon mucosa was determined by immunohistochemistry and Real-Time PCR. Result:Rats in model groups exhibited typical DRS with sign of hyperaemia

edema

hemorrhagic spots and bleeding in the gastric mucosa compared to control. The expression of AQP4 in colon musoca significantly decreased in DRS group compared to control group(P<0.05).Conversely

exposure of DRS rats to Huoxiang Zhengqi liquid resulted in increase in expression in AQP4 in the colon mucosa(P<0.05 versus DRS group). Conclusion:Huoxiang Zhengqi liquid could have the effect of curing the DRS-induced diarrhoea

increased expression of AQP4 may play an important role.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effects of Huoxiang Zhengqi Liquid on Expression of ZO-1 in Ileum Mucosa of Rats with Dampness Retention Syndrome

Effects of Huoxiang Zhengqi Liquid on the Anti-oxidation and Expression of EGFR in Gastric Mucosa of Rats with Dampness Retention Syndrome

Effects of Huashi Liquid on Content of -xylose and Ghrelin inSerum of Rats with Dampness Retention Syndrome

Related Author

HUANG Xue-kuang

Related Institution

No data

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰