Objective:To observe the effect of Tripterygium granule on expression of bone marrow CD4+/CD8+T-lymphocytes in mice aplastic anemia model. Method:A mouse model of aplastic anemia was established and divided into five groups

normal group and model group (fed with normal saline)

compound Tripterygium granule group (10.63

13.29 g·kg-1

ig)

testosterone undecanoate group (22 mg·kg-1

ig). After continuous administration of 35 d

bone marrow mononuclear cells were separated and cultured for 24 h. Expression levels of CD4+

CD8+

CD4+/CD8+T cells were analyzed by flow cytometry. Result:Compared with the normal group

amount of CD4+

CD8+

CD4+/CD8+T cells was decreased in model group

which of CD4+

CD4+/CD8+T cells were significantly lower than the normal group (P<0.05)

CD4+and CD8+were decreased

while CD4+/CD8+was increased in testosterone undecanoate group

but there was no significant difference compared with model group. Compared with the model group

amount of CD4+

CD8+

CD4+/CD8+in high and low dose group of compound Tripterygium granule was increased

which of CD4+

CD4+/CD8+T cells was significantly different from model group and testosterone undecanoate group (P<0.05). Conclusion:Compound Tripterygium granule can regulate the abnormal state of bone marrow T cells

restore balance between T cells and T cell subsets

and promote the recovery of bone marrow function.