Objective: To study the effect of Danggui Buxue decoction(DBD) on proliferation in tumor vascular endothelial cells

and to explore the mechanism of inhibition of tumor angiogenesis. Method: After different doses of DBD acting on vascular endothelial cells (EA. Hy926) co-cultured with the tumor cells (A549) model

CCK-8 assay was used to observe the proliferation of vascular endothelial cells co-cultured with the tumor cells; enzyme-cinked immunosorbant assay (ELISA) was used for assay of vascular endothelia growth factor (VEGF) and its receptors (VEGFR1

VEGFR2

sVEGFR1

sVEGFR2) and expression of vascular endothelial cells co-cultured with the tumor cells. Result: Compared with normal group

vascular endothelial cells co-cultured with tumor cells proliferation capacity increased (P<0.01); DBD each dose group could promote the proliferation of normal endothelial cells

inhibit the proliferation of vascular endothelial cells co-cultured with tumor cells with a manner of dose and effect

compared with model group

there was significant difference (P<0.01). DBD each dose could promote vascular endothelial cell co-cultured with tumor cells expression of VEGF

compared with model group

co-cultured of high dose group and dose group showed a significantly different (P<0.01). Co-culture model group was significantly up-regulate VEGFR1

VEGFR2 expression

the groups DBD inhibited the expression of VEGFR1

VEGFR2 and showed a dose relationship. Co-cultured with tumor vascular endothelial cells in model group decreased expression of sVEGFR1 and sVEGFR2

each dose group of DBD could promote sVEGFR1 and sVEGFR2 expression

showed a dose relationship. Conclusion: DBD can Inhibit proliferation of vascular endothelial cells co-cultured with the tumor cells. The mechanism might be related to regulation of VEGF and its receptors