Simultaneous Determination of Eight Major Compounds in Mailuoning Injection by HPLC

WU Ting; ZHANG Jun; ZHOU Ling; JU Wen-zheng; TAN Heng-shan

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Simultaneous Determination of Eight Major Compounds in Mailuoning Injection by HPLC

更新时间：2024-01-04

- Simultaneous Determination of Eight Major Compounds in Mailuoning Injection by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 22, Pages: 101-105(2012)
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- Published：2012
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WU Ting, ZHANG Jun, ZHOU Ling, et al. Simultaneous Determination of Eight Major Compounds in Mailuoning Injection by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(22): 101-105.
DOI：

WU Ting, ZHANG Jun, ZHOU Ling, et al. Simultaneous Determination of Eight Major Compounds in Mailuoning Injection by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(22): 101-105. DOI：

摘要

目的: 建立用高效液相色谱法梯度洗脱同时测定脉络宁注射液中绿原酸、新绿原酸、隐绿原酸、咖啡酸、肉桂酸、3

4-二-O-咖啡酰基奎宁酸

5-二-O-咖啡酰基奎宁酸

5-二-O-咖啡酰基奎宁酸含量的方法

并对3个批次脉络宁注射液中上述8种化合物进行定量分析。方法: 流动相乙腈-0.8%醋酸

梯度洗脱

流速1 mL·min-1

柱温30 ℃

检测波长λ1=327 nm(绿原酸、新绿原酸、隐绿原酸、咖啡酸、3

4-二-O-咖啡酰基奎宁酸

5-二-O-咖啡酰基奎宁酸

5-二-O-咖啡酰基奎宁酸)

λ2=278 nm(肉桂酸);进样量为10 μL。结果: 绿原酸、新绿原酸、隐绿原酸、咖啡酸、肉桂酸、3

4-二-O-咖啡酰基奎宁酸

5-二-O-咖啡酰基奎宁酸

5-二-O-咖啡酰基奎宁酸的线性范围分别为2.57~25.7(r=0.999 7)

2.05~20.5(r=0.999 7)

2.26~22.6(r=0.999 9)

1.92~19.2(r=0.999 9)

1.16~11.6(r=0.999 7)

2.282~22.82(r=0.999 8)

1.839~18.39(r=0.999 8)

2.194~21.94(r=0.999 7)mg·L-1

加样回收率分别为95.26%(RSD 1.21%)

96.03%(RSD 1.09%)

97.25%(RSD 2.18%)

100.98%(RSD 1.98%)

97.44%(RSD 2.38%)

97.78%(RSD 2.24%)

98.37%(RSD 2.15%)

95.82%(RSD 2.30%)。结论: 方法简便、快捷

准确、重复性好

可为脉络宁注射液提供质量控制依据。

Abstract

Objective:To establish an HPLC method for determination of eight major compounds in Mailuoning injection

including chlorogenic acid

neochlorogenic acid

cryptochlorogenic acid

caffeic acid

cinnamic acid

4-dicaffeoylquinic acid

5-dicaffeoylquinic acid and 4

5-dicaffeoylquinic acid. Method: The mobile phase was composed of acetonitrile-0.8% acetic acid with gradient elution. The flow rate was 1.0 mL·min-1. The column temperature was set at 30 ℃. The detection wavelength was set at 327 nm for chlorogenic acid

neochlorogenic acid

cryptochlorogenic acid

caffeic acid

4-dicaffeoylquinic acid

5-dicaffeoylquinic acid and 4

5-dicaffeoylquinic acid and 278 nm for cinnamic acid. The injection volume was 10 μL. Result: The linear ranges of eight compounds were as follows: 2.57-25.7 mg·L-1(r=0.999 7) for chlorogenic acid

2.05-20.5 mg·L-1(r=0.999 7) for neochlorogenic acid

2.26-22.6 mg·L-1(r=0.999 9) for cryptochlorogenic acid

1.92-19.2 mg·L-1(r=0.999 9) for caffeic acid

1.16-11.6 mg·L-1(r=0.999 7) for cinnamic acid

2.282-22.82 mg·L-1(r=0.999 8) for 3

4-dicaffeoylquinic acid

1.839-18.39 mg·L-1(r=0.999 8) for 3

5-dicaffeoylquinic acid and 2.194-21.94 mg·L-1(r=0.999 7) for 4

5-dicaffeoylquinic acid. The average recoveries were 95.26%(RSD 1.21%)

96.03%(RSD 1.09%)

97.25%(RSD 2.18%)

100.98%(RSD 1.98%)

97.44%(RSD 2.38%)

97.78%(RSD 2.24%)

98.37%(RSD 2.15%)

95.82%(RSD 2.30%)

respectively. Conclusion: The method is convenient

rapid and accurate with good reproducibility and could be used for the quality control of Mailuoning injection.

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