Objective:To investigate feasibility of extraction and concentration technology for protein from Buthus martensii rapidly by wet superfine grinding-ultrafiltration method. Method: Pharmacodynamic difference of protein from B. martensii was compared by in vivo anticoagulant and in vivo antithrombotic experiments in rats

which were prepared by two different extraction technology(wet superfine grinding-ultrafiltration method and pepsin enzymatic hydrolysis method)

in order to investigate feasibility of extraction and concentration for protein from B. martensii by wet superfine grinding-ultrafiltration method. Result: Protein from B. martensii through two kinds of extraction methods could both significantly prolong blood coagulation time and plasma APTT

PT

TT of rats.Furthermore

they could reduce dry and wet weight of rat arteriovenous thrombosis significantly

compared with physiological saline control group

there were statistically significant difference. Conclusion: After approved by in vivo anticoagulant and in vivo antithrombotic experiments in rats

it was feasible of wet superfine grinding-ultrafiltration method used to extract and concentrate protein from B. martensii rapidly.