Effects of Tangluoning Formula on Gene Expression of MnSOD, CuZnSOD and GPx in Sciatic Nerve of Diabetic Rats
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Effects of Tangluoning Formula on Gene Expression of MnSOD, CuZnSOD and GPx in Sciatic Nerve of Diabetic Rats
Chinese Journal of Experimental Traditional Medical FormulaeVol. 19, Issue 1, Pages: 175-179(2013)
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Published:2013
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ZOU Da-wei, GAO Yan-bin, ZHU Zhi-yao, et al. Effects of Tangluoning Formula on Gene Expression of MnSOD, CuZnSOD and GPx in Sciatic Nerve of Diabetic Rats[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(1): 175-179.
DOI:
ZOU Da-wei, GAO Yan-bin, ZHU Zhi-yao, et al. Effects of Tangluoning Formula on Gene Expression of MnSOD, CuZnSOD and GPx in Sciatic Nerve of Diabetic Rats[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(1): 175-179.DOI:
Effects of Tangluoning Formula on Gene Expression of MnSOD, CuZnSOD and GPx in Sciatic Nerve of Diabetic Rats
Objective:To explore possible molecular mechanism of Tangluoning(TLN) formula in improving oxidative stress. Method: The SD rats were divided into control group
the others were given intraperitoneal injection of streptozocin(STZ) (60 mg·kg-1) after 12 hours of fasting to build diabetic model. The rats with blood glucose level being more than 16.7 mmol·L-1 after stop 8 hours of fasting were regarded as modelling successfully after 3 days.The rats modeling successfully were randomly divided into the model group
TLN group and alpha-lipoic acid group (LA)
15 in each group. Drugs were administrated orally for 8 weeks after the STZ treatment.Blood glucose
cool or thermal response time
thermal threshold were determined during the experimental process. After eight weeks of treatment
Real-time PCR technology was employed to measure Mn superoxide dismutase(SOD) mRNA
CuZnSOD mRNA and glutathione peroxidase(GPx) mRNA expression in the sciatic nerve. Result: The expression of MnSOD mRNA
CuZnSOD mRNA and GPx mRNA was increased after STZ treatment. Compared with the model group
the cool or thermal response time was decreased
the thermal threshold was increased
the gene expression of MnSOD
CuZnSOD and GPx was increased by TLN. Conclusion: Tangluoning formula could improve hyperalgesia
dysesthesia
increase antioxidant enzymes gene expression to improve oxidative stress.
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Related Institution
College of Integrated Traditional Chinese and Western Medicine,Hebei University of Chinese Medicine,Hebei Key Laboratory of Integrated Medicine on Liver-kidney Patterns
International Joint Research Center for Diabetes Prevention and Treatment with TCM, Ministry of Science and Technology
Key Laboratory of TCM Health Sciences, Ministry of Education, Beijing University of Chinese Medicine
College of Traditional Chinese Medicine (TCM), Beijing University of Chinese Medicine
School of Life Sciences,Beijing University of Chinese Medicine