Objective:To investigate attenuating toxicity mechanism of processing for Arisaema erubescens. Method: Toxicity of raw products

processed products

raphides and different extraction site of A. erubescens were compared by acute toxicity test in mice and rabbit conjunctival irritation test;Change condition of special calcium oxalate raphides and impact of related ion from alum on morphological change of raphides before and after processing of A. erubescens were observed by scanning electron microscopy. Result: Acute toxicity test results showed that LD50 of raw products powder group

processed products powder group

raphides group of A. erubescens were 1062

2788

42.53 mg·kg-1.Results of rabbit conjunctival irritation test showed that raw powder group and raphides group had low and moderate irritation

but other various extracts had no irritation.Electron microscopy showed that ultrastructure of raphides occurred significantly change before and after processing of A. erubescens

and it was destroyed after soaked in KAl (SO4)2·12H2O or AlCl3 solution for 12 h

but no big change after soaked in Na2SO4 or KCl solution

the tip still existed. Conclusion: Raphides from A. erubescens was main material foundation of irritating toxicity

irritation toxicity of raphides from A. erubescens was related with its morphology

mechanism of attenuating toxicity of processed A. erubescens by alum was that Al3+ may accelerate destruction of calcium oxalate raphides morphology.