Simultaneous Determination of Baicalin and Emodin in Liyan Jiedu Granules by HPLC

LIU A-jing; QI Guang-cai; LIU Zhen-ye; BAI Zi-jing

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Simultaneous Determination of Baicalin and Emodin in Liyan Jiedu Granules by HPLC

更新时间：2024-01-04

- Simultaneous Determination of Baicalin and Emodin in Liyan Jiedu Granules by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 4, Pages: 146-149(2013)
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- Published：2013
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LIU A-jing, QI Guang-cai, LIU Zhen-ye, et al. Simultaneous Determination of Baicalin and Emodin in Liyan Jiedu Granules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(4): 146-149.
DOI：

LIU A-jing, QI Guang-cai, LIU Zhen-ye, et al. Simultaneous Determination of Baicalin and Emodin in Liyan Jiedu Granules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(4): 146-149. DOI：

摘要

目的: 建立同时测定利咽解毒颗粒中黄芩苷和大黄素含量的HPLC法并通过响应曲面优化法对二者的提取工艺进行优化。方法: 采用Agilent ZORBAX SB-C18 (4.6 mm×250 mm

5 μm)色谱柱

以乙腈(A)-0.2%磷酸溶液(B)为流动相梯度洗脱

流速1.0 mL·min-1

检测波长为280 nm和245 nm

柱温28℃。结果: 黄芩苷和大黄素分别在2.016~134.4

0.408 0~27.20 mg·L-1呈良好的线性关系; 平均回收率(n=6)为 98.9%和97.8%。结论: 方法简便、准确、重复性好

具有较强的实用价值。

Abstract

Objective: To develop an HPLC method for the determination of baicalin and emodin in Liyan Jiedu granules

response surface methodology (RSM) was applied to predict optimum conditions for extraction. Method: The analysis was carried out on an Agilent ZORBAX SB-C18 column (4.6 mm×250 mm

5 μm). The mobile phase consisted of acetonitrile (A) and 0.2% phosphoric acid (B) with gradient elution at a flow rate of 1.0 mL·min-1. The detection wavelength were set at 280

245 nm

respectively. The column temperature was 28℃. Result: The linear ranges of baicalin and emodin were 2.016-134.4 and 0.408 0-27.20 mg·L-1

respectively. The average recoveries (n=6) of these were 98.9% and 97.8%

respectively. Conclusion: This method is simple

accurate and repeatable

and can be used for the quality control of Liyan Jiedu granules.

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Related Author

WANG Hou-wei

GAO De-min

TIAN Jing-zhen

LI Xiao-ming

LUO Yu-jian

YUAN Yuan

HUANG Lu-qi

ZHANG Wei-hua

Related Institution

Department of Pharmacy,First Affiliated Hospital of PLA General Hospital

School of Traditional Chinese Medicine,Capital Medical University

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

College Pharmacy of Xinjiang Medical University

The First Affiliated Hospital of Guiyang College of Traditional Chinese Medicine

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