Determination of Homoplantaginin Content in  Extract by HPLC

GE Ting-ting; CHENG Jian-ming

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Determination of Homoplantaginin Content in Extract by HPLC

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- Determination of Homoplantaginin Content in Extract by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 23, Pages: 59-61(2012)
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- Published：2012
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GE Ting-ting, CHENG Jian-ming. Determination of Homoplantaginin Content in Extract by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(23): 59-61.
DOI：

GE Ting-ting, CHENG Jian-ming. Determination of Homoplantaginin Content in Extract by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(23): 59-61. DOI：

摘要

目的: 建立荔枝草提取物中高车前苷含量的高效液相(HPLC)测定方法。方法: Hedera ODS-2 色谱柱(4.6 mm×250 mm

5 μm)

流动相甲醇-0.5%冰醋酸水溶液(50∶50)

柱温30 ℃

流速1 mL·min-1

检测波长335 nm。结果: 高车前苷在0.20~2.01 μg呈良好的线性关系

平均回收率101.70%

RSD 1.84%。结论: 该方法简便、准确、专属性强

可以作为荔枝草提取物中高车前苷含量测定的一种有效方法。

Abstract

Objective: To establish the assay method for determining content of homoplantaginin in extract of Salvia plebeia. Method : Hedera ODS-2 column was used with methanol-0.5% acetic acid (50∶50) as mobile phase; the column temperature was maintained at 30 ℃; the flow rate was 1 mL·min-1; detection wavelength was set at 335 nm. Result: Homoplantaginin showed a good linear relationship within 0.20-2.01 μg. The average of coefficient of recovery was 101.70%

RSD was 1.84%. Conclusion: This method is simple

accurate

specific

and can be considered as an effective method for determining content of homoplantaginin in the extract of Salvia plebeia.

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Related Institution

Henan Engineering Research Center for Quality Control and Evaluation of Traditional Chinese Medicine（TCM），School of Pharmacy，Henan University of Chinese Medicine

Luohe Medical College

Third Affiliated Hospital of Henan University of Chinese Medicine

Tianjin University of Traditional Chinese Medicine

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

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