Correlation Study of Free and Total Ferulic Acid Content in Dried and Fresh

LIN Li; JIN Ling; LI Ying-dong; HOU Jia; GAO Su-fang; YANG Jian-cheng

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Correlation Study of Free and Total Ferulic Acid Content in Dried and Fresh

更新时间：2024-01-04

- Correlation Study of Free and Total Ferulic Acid Content in Dried and Fresh
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 22, Pages: 94-97(2012)
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- Published：2012
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LIN Li, JIN Ling, LI Ying-dong, et al. Correlation Study of Free and Total Ferulic Acid Content in Dried and Fresh [J]. Chinese journal of experimental traditional medical formulae, 2012, 18(22): 94-97.
DOI：

LIN Li, JIN Ling, LI Ying-dong, et al. Correlation Study of Free and Total Ferulic Acid Content in Dried and Fresh [J]. Chinese journal of experimental traditional medical formulae, 2012, 18(22): 94-97. DOI：

摘要

目的: 采用HPLC测定当归鲜品与干品中游离阿魏酸和总阿魏酸的含量

并对其进行比较。方法: ZORBAX Eclipse XDB-C18(4.6 mm ×250 mm

5 μm)分析柱

流动相乙腈-0.085 %磷酸(17∶83)

检测波长316 nm

柱温35 ℃

流速 1.0 mL·min-1

进样量为10 μL。测定样品中游离阿魏酸和总阿魏酸的含量。结果: 游离阿魏酸和总阿魏酸分别在0.024 2~0.121 0 μg和0.0242~0.192 36 μg呈良好的线性关系。回收率分别为96.34%

97.35%;09年当归干品中游离阿魏酸含量最高

鲜品中总阿魏酸含量最高。结论: 该方法准确简便

具有相对良好的重复性和稳定性

有利于提高当归药材的质量控制。

Abstract

Objective:The free and total ferulic acid content in dried and fresh Angelica sinensis Radix was measured by HPLC. Method: Free and total ferulic acid content was determined by HPLC. The separation was performed on a ZORBAX Eclipse XDB-C18 column (4.6 mm×250 mm

5 μm) with acetonitrile-0.085% phosphoric acid (17∶83) as mobile phase at temperature of 35 ℃. The flow rate was 1.0 mL·min-1 and the detected wavelength was set at 316 nm; the injection volume was 10 μL. Result: The linear relationship of free and total ferulic acid was excellent within the range of 0.024 2-0.121 0 μg and 0.024 2-0.192 36 μg

and the recovery rates were 96.34% and 97.35%

respectively. The free ferulic acid in dried A. sinensis Radix of 2009 was the highest and the total ferulic acid in fresh A. sinensis Radix was the highest. Conclusion: This method was simple and accurate with good repeatability and stability. It will help to improve the quality control of A. sinensis Radix.

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Related Institution

Department of Pharmacy,Guiyang College of Traditional Chinese Medicine

Jiangsu Key Laboratory for TCM Formulae Research,Nanjing University of Chinese Medicine

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