Objective: To establish a HPLC method for the simultaneous determination of six alkaloids (dehydrocheilanthifoline

protopine

corynoline

acetylcorynoline

oxysanguinarine

dihydrosanguinarine) in Corydalis bungeana. Method: The chromatographic separation was performed on a Kromasil C18 column(4.6 mm×250 mm

5 μm) at room temperature with the 0.02 mol ·L-1 KH2PO4-methanol as the mobile phrase in gradient elution. The detection wavelength was set at 289 nm and the flow rate was 0.8 mL·min-1. Result: All the compounds were linear(r>0.999)in the range of the test concentrations

and the average recovery of the method was between 98.26% and 102.10%

RSD<3.0%. Conclusion: The method established by this paper can be used to determine six alkaloid components in C. bungeana simultaneously

and provides a scientific basis for the quality evaluation of C. bungeana.