Objective:To observe the effects and mechanism of the serum containing Buyang Huanwu decoction on endothelial dysfunction induced by homoeysetine(Hcy) in human umbilical vein endothelial cell. Method: Human umbilical vein endothelial cells (Eahy926) were incubated with Hcy.Cell viability

the activity of superoxide dismutase(SOD)

lactate dehydrogenase(LDH)

and the content of malondialdehyde (MDA) in the medium were determined and the concentrations of sICAM-1(by ELISA)

TNF-α(by ELISA) in the conditioned medium. The activity of NF-κB was determined by fluorescence microscopy and the expression of I-κBα protein were measured via Western-blot. Result: Incubation of Eahy926 with different concentrations of the serumcontaining Buyang Huanwu decoction (5%

10%

20%)enhanced cell viability. Pretreatment with the serum containing Buyang Huanwu decoction 10%

20% group improved activity of SOD·mL-1] compared with the model group (11.44±1.00) U·mL-1

P<0.01;decreased the levels of LDH

MDA(1.62±0.06)

(1.47±0.18)nmol·L-1

compared with the model group (1.89±0.25) nmol·L-1

P<0.01; down-regulated the content of sICAM-1(0.93±0.11)

(1.00±0.14) μg·L-1and TNF-α(63.71±2.64)

(66.22±4.43)ng·L-1

compared with the model group(2.32±0.23) μg·L-1

(125.67±2.69)ng·L-1

P<0.05;as well as markedly inhibited the increased activity of NF-κB. Conclusion: The serum containing Buyang Huanwu decoction could prevent endothelial dysfunction induced by Hcy and inhibition the activity of NF-κB may be its mechanism of preventing.