Objective: The factorial design method of effective parts of Astragalus 2×2×2 (Astragalus flavonoids

polysaccharides of astragalus and Astragalus saponin) was used analyze intervention of effect and mechanism in hemorrhagic anemia model mice and their interaction. Method: One hundred and eight mouse randomly were divided into 9 groups

12 each group. In addition to normal group

orbital venous bleeding was executed at 1

4

7 day

rasprctively

bloodletting is 0.5 mL·20 g-1

normal groups

model groups and intragastric infusion were given with distilled water 10 g·kg-1

other groups were given corresponding drugs with dose of 0.166

0.445

0.611

0.069

0.235

0.514

0.680 g·kg-1. Factorial design analysis of variance methods was used to analyze peripheral hemoglobin (Hb) concentration

red blood cell (RBC) count

hematocrit (Hct)

hematopoietic factors such as indicators for analysis of variance. Result: Compared with normal group

peripheral blood Hb

RBC

Hct

erythropoietin(EPO)

serum transcription factor(GATA-1) in serum and kidney tissue

bone marrow

such as stem cell factor(SCF) index differences are statistically significant in model group(P<0.05). Flavone

polysaccharide and saponins glycoside had an obvious effect on peripheral blood (P<0.05)

combined application of flavone and saponins glycoside and polysaccharide and saponins glycoside showed a better action than single medication; flavone and polysaccharide had influence on serum EPO

GATA-1 and bone marrow SCF concentration

polysaccharide and saponins glycoside had effect on kidney organization EPO concentration. Conclusion: Combination of polysaccharides and saponins can improve peripheral blood in anemia mice picture.