Objective:To establish an HPLC method for determination of mesaconitine

hypaconitine

aconitine in Bitong medicated wine. Method: A Agela Promosil-C18 (4.6 mm×250 mm

5 μm) column was adopted

the mobile phase consisted of acetonitrile-tetrahydrofuran (25∶15

phase A)

0.1 mol·L-1 ammonium acetate(containing 0.5 mL glacial acetic acid per 1 000 mL

phase B) with gradient elution at the flow rate of 1.0 mL·min-1

and the detection wavelength was at 235 nm. Result: The linear range of mesaconitine was 0.118 9 -0.990 9 μg (r =0.999 9). The average recovery was 98.07% with RSD of 1.7%. The linear range of hypaconitine was 0.074 5-0.621 0 μg(r=0.999 9). The average recovery was 99.08% with RSD of 1.5%.The linear range of aconitine was 0.009 4-0.078 3 μg(r =0.999 9). The average recovery was 98.32% with RSD of 2.0%. Conclusion: The developed method was reliable

and can be used for the quality control of Bitong medicated wine.