Protection  of Epigallocatechin Gallate on Oxidative Stress Induced by Influenza Infection

WEI Fei; LING Jia-xin; LI Ning; LI Jin-lin; CHEN Liang-jun; XIONG Hai-rong; LUO Fan; LIU Yuan-yuan; XIAO Hong; YANG Zhan-qiu

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Protection of Epigallocatechin Gallate on Oxidative Stress Induced by Influenza Infection

更新时间：2024-01-04

- Protection of Epigallocatechin Gallate on Oxidative Stress Induced by Influenza Infection
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 24, Pages: 212-216(2012)
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- Published：2012
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WEI Fei, LING Jia-xin, LI Ning, et al. Protection of Epigallocatechin Gallate on Oxidative Stress Induced by Influenza Infection[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(24): 212-216.
DOI：

WEI Fei, LING Jia-xin, LI Ning, et al. Protection of Epigallocatechin Gallate on Oxidative Stress Induced by Influenza Infection[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(24): 212-216. DOI：

摘要

目的: 研究表没食子儿茶素没食子酸酯(EGCG)在体外对甲型流感病毒感染后氧化应激的影响

初步探讨EGCG抗流感病毒的发生机制。方法: 以1×104个细胞密度接种96 孔板

在体外用MTT法检测不同浓度EGCG对狗肾传代细胞(MDCK)的毒性浓度;病毒感染28 h后

用CPE观察法、血凝法、实时定量荧光PCR法评价EGCG对甲型流感病毒的抑制作用;并通过共聚焦显微技术和流式细胞术

探寻EGCG对甲型流感病毒感染后氧化应激的影响。结果: EGCG在体外能有效抑制甲型流感病毒的复制

病毒抑制率与感染后加药时间正相关(P<0.05);与病毒对照相比

20 mg·L-1 EGCG处理后流感病毒感染的MDCK细胞中活性氧的水平下降54%

继而有效抑制流感病毒引起的细胞凋亡。结论: EGCG主要抑制流感病毒的早期复制

可通过调控氧化应激

抑制流感病毒所致MDCK细胞凋亡。

Abstract

Objective:To investigate the molecular mechanism of the anti-influenza A virus effects of epigallocatechin gallate(EGCG) derived from green tea in vitro. Method: The cell viability of EGCG was determined by using MTT assay. And virus inhibitory rate was evaluated based on the observation of cytopathic effect (CPE)

hemagglutination assay and Real-time Quantitative PCR (QPCR). The mechanisms of action for the observed sensitivity of influenza A to EGCG at the molecular level was assessd by confocal microscope and flow cytometry. Result: Exposing Madin-Darby canine kidney (MDCK) cells with 20 mg·L-1 EGCG could inhibit influenza A replication in a time-dependent manner

probably by interrupting postbinding step. EGCG treatment could mitigate oxidative stress in influenza-infected cells and reduce the apoptosis induced by influenza infection. Conclusion: EGCG inhibits oxidative stress and apoptosis induced by influenza virus infection.

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