Determination of Aloperine in Different Parts of by HPLC

WEI Lei-chu; DENG Hong-zhu; Zhang Li; LIU Zhen-long; YI Yan-kui

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Determination of Aloperine in Different Parts of by HPLC

更新时间：2024-01-04

- Determination of Aloperine in Different Parts of by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 4, Pages: 140-143(2013)
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- Published：2013
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WEI Lei-chu, DENG Hong-zhu, Zhang Li, et al. Determination of Aloperine in Different Parts of by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(4): 140-143.
DOI：

WEI Lei-chu, DENG Hong-zhu, Zhang Li, et al. Determination of Aloperine in Different Parts of by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(4): 140-143. DOI：

摘要

目的: 建立苦豆子中苦豆碱的HPLC法;对苦豆子药材不同部位中苦豆碱含量进行考察

确定药材最佳采收部位。方法: 采用Kromasil NH2(4.6 mm×250 mm

5 μm)色谱柱

以乙腈-无水乙醇-3%磷酸水溶液(70:15:15)为流动相

检测波长205 nm

柱温25℃

流速1.0 mL·min-1。结果: 苦豆碱的线性范围为11.21~224.20 mg·L-1(r=0.999 9)

平均回收率(n=6)98.18%(RSD 4.1%);苦豆子叶、茎中苦豆碱含量分别为0.38%

0.02%

豆荚、花及种子中不含苦豆碱。结论: 该方法简便、准确、重复性好

可以作为苦豆子中苦豆碱的含量测定方法;研究结果显示苦豆碱在叶子中含量最高

可以将叶子作为提取分离苦豆碱的最佳药材部位。

Abstract

Objective: To establish a HPLC for determination of aloperine in Sophora alopcuroides and investigate the content of aloperine in different medicinal parts of S. alopcuroides to define an optimum harvest part. Method: The separation was carried out on a Kromasil NH2 column (4.6 mm×250 mm

5 μm); the mobile phase was acetonitrile-absolute alcohol-3% phosphonic acid water solution (70:15:15); the detective wavelength was set at 205 nm. The column temperature was at kept at 25℃; the flow rate was 1.0 mL·min-1. Result: The linear ranges of aloperine was 11.21-224.20 mg·L-1 (r=0.999 9) and the average recovery (n=6) was 98.18% (RSD 4.1%). The content of aloperine in leaf and stem was 0.38% and 0.02% respectively. However

aloperine was not detected in seed

legume and flower. Conclusion: The method is simple

accurate with good repeatability. It can be used as a method for determination of aloperine in S. alopcuroides; the results show that leaf has the highest content of aloperine. Hence

leaf is the optimum harvest part for aloperine extraction and separation.

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