Optimization of Extraction Technology of  and  from Fushubao Gel

YANG Rui; WANG Jian-chun; CHEN Xiao-feng; WANG Ming-zhi; ZHANG Yong-mei

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Optimization of Extraction Technology of and from Fushubao Gel

更新时间：2024-01-04

- Optimization of Extraction Technology of and from Fushubao Gel
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 2, Pages: 46-48(2013)
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- Published：2013
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YANG Rui, WANG Jian-chun, CHEN Xiao-feng, et al. Optimization of Extraction Technology of and from Fushubao Gel[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(2): 46-48.
DOI：

YANG Rui, WANG Jian-chun, CHEN Xiao-feng, et al. Optimization of Extraction Technology of and from Fushubao Gel[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(2): 46-48. DOI：

摘要

目的: 优选妇舒保凝胶中黄柏、苦参的提取工艺。方法: 以出固率和盐酸小檗碱、苦参碱的得率为指标

单因素试验考察水煎煮法、乙醇回流法及乙醇渗漉法对黄柏、苦参提取效率的影响;选取乙醇体积分数、乙醇用量、提取次数、提取时间为考察因素

采用正交试验优选妇舒保凝胶中黄柏、苦参的提取工艺。结果: 选择乙醇回流法进行提取

最佳提取工艺为加8倍量50%乙醇提取3次

每次1.5 h

总生物碱得率约2.47%。结论: 该优选工艺稳定可行、重复性良好

为妇舒保凝胶中有效成分的提取提供试验依据。

Abstract

Objective: To optimize extraction technology of Phellodendrom chinense and Sophora flavescens from Fushubao gel. Method: Taking yield of matrine

berberine hydrochloride and extract as indexes

single factor test was used to choose extraction method

including water boiling method

ethanol reflux method and ethanol percolation method;Orthogonal test was designed to optimize extraction technology of P. chinense and S. flavescens from Fushubao gel with extraction times

extraction time

the amount and concentration of ethanol as factors. Result: Ethanol reflux method was selected

optimum extraction technology was as follows:extracted 3 times with 8 times the amount of 50% ethanol

1.5 hours each time.Yield of total alkaloid was about 2.47%. Conclusion: This optimized process was stable

feasible and reproducible

it could provide experimental basis for extraction of active ingredients from Fushubao gel.

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