Study on Mice Spleen Lymphocyte Proliferation and Transformation Effect and Chemical Constituents of Xiangfu Siwu Decoction
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Study on Mice Spleen Lymphocyte Proliferation and Transformation Effect and Chemical Constituents of Xiangfu Siwu Decoction
Chinese Journal of Experimental Traditional Medical FormulaeVol. 18, Issue 23, Pages: 252-257(2012)
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Published:2012
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LIU Pei, DUAN Jin-ao, HONG Min, et al. Study on Mice Spleen Lymphocyte Proliferation and Transformation Effect and Chemical Constituents of Xiangfu Siwu Decoction[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(23): 252-257.
DOI:
LIU Pei, DUAN Jin-ao, HONG Min, et al. Study on Mice Spleen Lymphocyte Proliferation and Transformation Effect and Chemical Constituents of Xiangfu Siwu Decoction[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(23): 252-257.DOI:
Study on Mice Spleen Lymphocyte Proliferation and Transformation Effect and Chemical Constituents of Xiangfu Siwu Decoction
Objective: To evaluate the mice spleen lymphocyte proliferation and transformation effect of Xiangfu Siwu decoction(XFSWD) and explore the bioactive constituents. Method: The samples were prepared by macroporous adsorptive resins. mice spleen lymphocyte proliferation and transformation models were adopted to evaluate the immune related activities of XFSWD and its different separated fractions in vitro. The chemical constituents in the most active fraction were identified and determined by HPLC. Result: By mice spleen lymphocyte proliferation and transformation experiments
XFSWD
XFSW-3 and XFSW-7 showed high effect. Four main compounds including albiflorin
peoniflorin
ferulaic acid
senkyunolide I in active fraction XFSW-7 were identified and quantified by HPLC-DAD. The contents were 5.973%
5.902%
1.374% and 0.014%
respectively. Conclusion: Albiflorin
peoniflorin
ferulaic acid
senkyunolide I were the potential active constituents. XFSWD had the common material basis.
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