Therapeutic Effects of Microwave-assisted Extract of Xianling Gubao on Experimental Osteoporosis in Rats

ZHAO Hai-ping; Luo Yun; ZHANG Jing; LI Min; LIAO Zheng-gen; YANG Ming

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Therapeutic Effects of Microwave-assisted Extract of Xianling Gubao on Experimental Osteoporosis in Rats

更新时间：2024-01-04

- Therapeutic Effects of Microwave-assisted Extract of Xianling Gubao on Experimental Osteoporosis in Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 22, Pages: 183-186(2012)
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- Published：2012
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ZHAO Hai-ping, Luo Yun, ZHANG Jing, et al. Therapeutic Effects of Microwave-assisted Extract of Xianling Gubao on Experimental Osteoporosis in Rats[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(22): 183-186.
DOI：

ZHAO Hai-ping, Luo Yun, ZHANG Jing, et al. Therapeutic Effects of Microwave-assisted Extract of Xianling Gubao on Experimental Osteoporosis in Rats[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(22): 183-186. DOI：

摘要

目的: 探讨采用微波辅助提取技术(MAE)与传统提取工艺(TE)所得仙灵骨葆提取物的抗骨质疏松作用差异

为MAE的进一步推广及仙灵骨葆的临床应用提供资料。方法: 健康SD大鼠

随机分为正常对照组、模型组、福善美组、仙灵骨葆传统提取物中剂量组(1.0 g·kg-1)

微波辅助提取物低、中、高剂量组(0.33

1.0

3.0 g·kg-1)。上午除正常对照组外

各组采用维甲酸灌胃造模(80 mg·kg-1)

1次/d

共2周

同时下午除正常对照组与模型组外

各给药组灌胃给药

1次/d

共4周。给药结束后测定大鼠胫骨与股骨干骺端及腰椎的骨密度(BMD)。结果: 模型组与正常对照组比较

大鼠胫骨与股骨干骺端及腰椎骨BMD(0.267 5±0.011 9)

(0.228 9±0.015 2)

(0.247 1±0.012 6)g·cm-2

水平显著下降(P<0.01);与模型组比较

各给药组大鼠胫骨与股骨干骺端及腰椎骨BMD显著升高(P<0.05或P<0.01);与仙灵骨葆TE中剂量组(0.272 7±0.013 3)g·cm-2比较

仙灵骨葆MAE中剂量组大鼠胫骨干骺端BMD(0.283 3±0.020 0)g·cm-2水平明显升高(P<0.05);与福善美组比较

仅MAE高剂量组胫骨与股骨干骺端及腰椎骨BMD(0.290 1±0.014 3)

(0.251 5±0.006 7)

(0.261 7±0.007 9)g·cm-2水平与之未有明显差异。结论: 采用MAE所得的仙灵骨葆提取物抗骨质疏松作用明确

且具剂量依赖性;较之同等剂量的传统提取物

MAE提取物的抗骨质疏松作用呈增强趋势

今后应注重MAE在提升药物疗效与安全性等领域的研究。

Abstract

Objective:To compare the therapeutic effects of Xianling Gubao (XLGB) extracts by microwave-assisted extraction(MAE) and traditional extraction(TE) on osteoporosis

and to provide data for the further generalization of MAE and clinical application of XLGB. Method: Healthy SD rats were randomly divided into 7 groups: normal control group

osteoporosis model group

Fosamax group

middle-dose of XLGB by TE group(1.0 g·kg-1)

low-dose of XLGB by MAE group(0.33 g·kg-1)

middle-dose of XLGB by MAE group(1.0 g·kg-1)

high-dose of XLGB by MAE group(3.0 g·kg-1). In the morning all the rats except the normal control group were given retinoic acid (80 mg·kg-1)through intragastric administration for 2 weeks

at the same time in the afternoon all the rats except the normal control group and the osteoporosis model group were given corresponding drugs once per day for 4 weeks. After that

bone density (BMD) of tibial metaphysis

femoral metaphysis and lumbar were determined. Result: Compared with the normal control group

the BMD of tibial metaphysic (0.267 5±0.011 9) g·cm-2

femoral metaphysic (0.228 9±0.015 2) g·cm-2 and lumbar (0.247 1±0.012 6) g·cm-2 of the osteoporosis model group were obviously decreased (P<0.01); compared with the osteoporosis model group

the BMD of tibial metaphysis

femoral metaphysis and lumbar of all the administration group were obviously increased (P<0.05 or P<0.01); compared with middle-dose of XLGB by TE group(0.272 7±0.013 3) g·cm-2

the BMD of tibial metaphysis of middle-dose of XLGB by MAE group (0.283 3±0.020 0)g·cm-2 were obviously increased (P<0.05); only did the BMD of tibial metaphysic (0.290 1±0.014 3)g·cm-2

femoral metaphysic (0.251 5±0.006 7)g·cm-2and lumbar(0.261 7±0.007 9)g·cm-2 of the high-dose of XLGB by MAE group have no obvious differences with Fosamax group. Conclusion: The therapeutic effect of XLGB by MAE on osteoporosis was exact and dose dependent. The effect of XLGB by MAE was better than that by TE. It is suggested that more attention should be paid to the therapeutic effect and safety of MAE.

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