Isolation, Identification and Quantitative Analysis of Vitexin 2″---D-glucopyranoside in the Whole Herb of

FEI Jiao-dong; CHEN Lin; DING Gang; ZHANG Hong-wu; LI Rong-tao; YU Chang-yuan; ZOU Zhong-mei

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Isolation, Identification and Quantitative Analysis of Vitexin 2″---D-glucopyranoside in the Whole Herb of

更新时间：2024-01-04

- Isolation, Identification and Quantitative Analysis of Vitexin 2″---D-glucopyranoside in the Whole Herb of
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 1, Pages: 64-67(2013)
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- Published：2013
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FEI Jiao-dong, CHEN Lin, DING Gang, et al. Isolation, Identification and Quantitative Analysis of Vitexin 2″---D-glucopyranoside in the Whole Herb of [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(1): 64-67.
DOI：

FEI Jiao-dong, CHEN Lin, DING Gang, et al. Isolation, Identification and Quantitative Analysis of Vitexin 2″---D-glucopyranoside in the Whole Herb of [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(1): 64-67. DOI：

摘要

目的: 分离制备流苏金石斛药材指标性成分

并建立测定流苏金石斛中该成分含量的高效液相色谱法。方法: 采用Shiseido(Pak-C18)(4.6 mm×250 mm

5 μm)色谱柱

以乙腈-水(20:80)为流动相

流速1.0 mL·min-1

检测波长340 nm

柱温25℃。结果: 从流苏金石斛中分离制备其指标性成分牡荆素2″-O-β-D-葡萄糖苷;该成分在0.016~1.6 μg线性关系良好(r=0.999 8)

平均加样回收率为99.66%(RSD 1.05%)。结论: 方法快速简便

可靠

重复性好

为流苏金石斛药材的质量控制提供依据。

Abstract

Objective:To prepare chemical marker and establish HPLC method for determining this marker in the whole herb of Flickingeria fimbriata. Method: A Pak-C18(4.6 mm×250 mm

5 μm)column was adopted with a mobile phase of acetonitrile-water (20:80) at the flow rate of 1.0 mL·min-1 and the column temperature was set at 25℃. The detection wavelength was 340 nm. Result: Vitexin 2″-O-β-D-glucopyranoside was firstly isolated as a chemical marker from F. fimbriata. This marker showed a good linear relationship in the range of 0.016-1.6 μg(r=0.999 8)and the average recovery was 99.66% (RSD 1.05%). Conclusion: The method is quick

simple

reliable

and reproducible

which can be used for quality control of F. fimbriata.

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