Optimization of Purification Technology for Total Saponins from by Macroporous Resin
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Optimization of Purification Technology for Total Saponins from by Macroporous Resin
Chinese Journal of Experimental Traditional Medical FormulaeVol. 18, Issue 20, Pages: 55-57(2012)
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Published:2012
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BAO Xiao-hua, WANG Qing-hu, AO·WULIJI Nayintai-alamusi. Optimization of Purification Technology for Total Saponins from by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(20): 55-57.
DOI:
BAO Xiao-hua, WANG Qing-hu, AO·WULIJI Nayintai-alamusi. Optimization of Purification Technology for Total Saponins from by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(20): 55-57.DOI:
Optimization of Purification Technology for Total Saponins from by Macroporous Resin
Objective:To optimize refining process of total saponins from extracts of Astragalus membranaceus. Method: With total saponins from A. membranaceus as index
the content of it was determined by UV spectrophotometry
adsorption capacity
kind and its amount of elution solvent were investigated by single factor test; Sample quantity
adsorption time and elution flow velocity were chosen as factors
elution conditions were optimized by orthogonal test. Result: optimized refining process was:mixed with 5 mL sample solution and 10 mL water
on column
adsorption time 1.0 h
eluted with 10 times the amount of water and 5 times the amount of 70% ethanol
collected eluent
elution flow velocity 2 mL·min-1.Eluent was decompressed recovery to dried
purity of total saponins was up to 87.63% after drying at 105 ℃. Conclusion: This optimized technology was simple and feasible
it could be better to purify total saponins from A. membranaceus.
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