Study on the Relative Recovery of Chlorine-e6 in Microdialysis Probe and its Influence Factors

XU Yue-yuan; LING Jia-jun; XIE Yi; WANG An-yuan

您当前的位置：

首页 >

文章列表页 >

Study on the Relative Recovery of Chlorine-e6 in Microdialysis Probe and its Influence Factors

更新时间：2024-01-04

- Study on the Relative Recovery of Chlorine-e6 in Microdialysis Probe and its Influence Factors
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 24, Pages: 120-124(2012)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2012
- 稿件说明：
移动端阅览
XU Yue-yuan, LING Jia-jun, XIE Yi, et al. Study on the Relative Recovery of Chlorine-e6 in Microdialysis Probe and its Influence Factors[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(24): 120-124.
DOI：

XU Yue-yuan, LING Jia-jun, XIE Yi, et al. Study on the Relative Recovery of Chlorine-e6 in Microdialysis Probe and its Influence Factors[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(24): 120-124. DOI：

摘要

目的: 建立二氢卟吩e6微透析探针相对回收率(relative recovery

RR)的测定方法

并考察流速、浓度、温度、时间对RR的影响

为体内微透析研究提供实验依据。方法: 利用浓度差法(增量法、减量法)进行二氢卟吩e6微透析探针体外回收率的比较研究

采用高效液相色谱仪(HPLC)测定二氢卟吩e6微透析取样后的浓度

利用探针回收率公式计算探针的RR。结果: 在同一浓度下

两种方法测定RR均随流速的增加而减少;增量法和减量法测得的RR一致;在同一流速下

浓度对RR的影响不大;RR在12 h内保持稳定;RR随温度的上升明显增加。结论: 确定了影响微透析体外回收率的因素

利用减量法测定的探针回收率与增量法接近

反向透析法可作为体内微透析研究中的二氢卟吩e6回收率的测定方法。

Abstract

Objective:To establish a method for the relative recovery (RR) of chlorine-e6 in microdialysis probe and to investigate the effect of the concentration

time and temperature on RR in chlorin-e6 microdialysis probe. Method: Gain method and loss method were used for the study. The concentration of chlorine-e6 in micro-dialysate samples was detected by HPLC and calculated probe RR with the formula used for the probe. Result: At the same concentration

RR decreased with the increase of the flow rate in the two methods. RR detected by gain method was similar to that detected by loss method. At the same flow rate

the concentration had no obvious effect on RR. RR kept steady within 12 hours

and increased obviously with the temperature. Conclusion: The influencing factors on RR in chlorin-e6 microdialysis probe are confirmed. The probe recovery obtained by gain method is similar to that by loss method. Retrodialysis can be used for the determination of the chlorine-e6 in-vivo recovery.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

No data

Related Author

No data

Related Institution

No data

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰