Effect of Extract on the Invasion and Migration in Human Gastric Carcinoma MGC-803 Cells

ZHU Yao-dong; QIAN Ya-yun; MA Hui; GUO Shi-yu; Hisamits Tadashi; LIU Yan-qing

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Effect of Extract on the Invasion and Migration in Human Gastric Carcinoma MGC-803 Cells

更新时间：2024-01-04

- Effect of Extract on the Invasion and Migration in Human Gastric Carcinoma MGC-803 Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 22, Pages: 180-183(2012)
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- Published：2012
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ZHU Yao-dong, QIAN Ya-yun, MA Hui, et al. Effect of Extract on the Invasion and Migration in Human Gastric Carcinoma MGC-803 Cells[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(22): 180-183.
DOI：

ZHU Yao-dong, QIAN Ya-yun, MA Hui, et al. Effect of Extract on the Invasion and Migration in Human Gastric Carcinoma MGC-803 Cells[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(22): 180-183. DOI：

摘要

目的: 探讨南蛇藤总萜对人胃癌MGC-803细胞侵袭和迁移能力的影响

并对其机制进行初步探讨。方法: 体外培养MGC-803细胞

分为南蛇藤总萜组(质量浓度分别为20

80 mg·L-1)、阴性对照组和阳性对照组

采用MTT法检测南蛇藤总萜对MGC-803细胞增殖的抑制作用;采用Transwell小室法检测南蛇藤总萜处理MGC-803细胞24 h后

细胞侵袭和迁移能力的改变;Western blotting法检测处理24 h后基质金属蛋白酶(MMP)-2和MMP-9的表达情况。结果: 南蛇藤总萜能抑制MGC-803细胞的增殖

呈一定的浓度及时间依赖性;南蛇藤总萜40

80 mg·L-1能显著降低MGC-803细胞的穿膜细胞数(P<0.01)

并呈浓度依赖性;南蛇藤总萜能降低MGC-803细胞中MMP-2和MMP-9蛋白的表达水平

并有浓度依赖性。结论: 南蛇藤总萜能抑制人胃癌MGC-803细胞的侵袭和迁移能力

其机制可能与抑制MMP-2

MMP-9的表达有关。

Abstract

Objective:To study the effect and mechanism of Celastrus orbiculatus Thunb. extract (COE) on invasion and migration of human gastric carcinoma MGC-803 cells. Method: The human gastric carcinoma MGC-803 cells were divided into negative and positive control group and COE groups (20

80 mg·L-1

respectively). The cytotoxicity effect was measured by using the MTT assay. The invasion and migration ability of MGC-803 were assayed by transwell assay. The expression levels of matrix metalloproteinase(MMP)-2 and MMP-9 in MGC-803 were examined by western blot assay. Result: COE suppressed the proliferation of MGC-803 cells in a time and does-dependent manner. The concentrations of 40

80 mg·L-1 of COE significantly inhibited the invasion and migration activity of MGC-803 cells. In addition

the expression levels of MMP-2

MMP-9 were dramatically suppressed by COE in a concentration-dependent manner. Conclusion: COE could obviously inhibit the migration and invasion ability of MGC-803 cells. Its mechanism may be related to the down-regulated expression of MMP-2 and MMP-9.

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