Objective: To establish a determination method of eugenol in traditional Mongolian medicine Shunqi Buxin-11 Pill. Method: High performance liquid chromatography (HPLC) method was applied with Hypersil ODS C18 (4.6 mm×250 mm

5 μm) column by methanol-water- phosphoric acid (65: 35: 0.05) as mobile phase with the flow rate of 1.0 mL·min-1; the column temperature was maintained at 30℃

and the UV detection wavelength was set at 283 nm. Result: Eugenol was linear in the range of 0.025-0.150 μg

r=0.999 8)

the average recovery was 96.94%

RSD was 1.95% (n=6). Conclusion: Precision of the method was high

and separating was good

and the method can be used for determination of eugenol in traditional Mongolian medicine Shunqi Buxin-11 Pill.