Objective: To observe the effect of botulinum toxin type A (BTX-A)

electrical field stimulation(EFS)and acetylcholine(ACh) on spontaneous contractility in gastric body and gastric fundus smooth muscle. Method: Muscle strips in gastric body and gastric fundus were prepared

and subdivided randomly into control group

EFS group

BTX-A(10 U·mL-1)group

BTX-A(10 U·mL-1)+ EFS group

ACh(100 μmol·L-1)group

ACh(100 μmol·L-1)+BTX-A(10 U·mL-1)group

Ach(100 μmol·L-1)+Atropine(1 μmol·L-1)group. The data were recorded by physiological experimental system of BL-420. Result: EFS enhanced the tension(P<0.05)and amplitude(P<0.01)in gastric body contractility

and similar results was observed in gastric fundus contractility; BTX-A decreased spontaneous contractile tension and amplitude(P<0.01)in gastric body and tension(P<0.05)in gastric fundus; BTX-A inhibited EFS-induced smooth muscle contractility including tension and amplitude(P<0.01)in gastric body

tension(P<0.01) and amplitude(P<0.05)in gastric fundus

BTX-A inhibited ACh-induced smooth muscle contractility including tension and amplitude(P<0.01)in gastric body and gastric fundus. Conclusion: EFS enhances smooth muscle spontaneous contractility in gastric body and gastric fundus; BTX-A inhibits gastric body and gastric fundus smooth muscle spontaneous contractility; BTX-A inhibits EFS and ACh-induced smooth muscle contractility in gastric body and gastric fundus.