Determination of Baicalin in Xiaoerqingfeihuatan Granule by HPLC

TANG Hui-hui; CAI Qing-yu; ZHANG Li-juan

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Determination of Baicalin in Xiaoerqingfeihuatan Granule by HPLC

更新时间：2024-01-04

- Determination of Baicalin in Xiaoerqingfeihuatan Granule by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 21, Pages: 112-114(2012)
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- Published：2012
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TANG Hui-hui, CAI Qing-yu, ZHANG Li-juan. Determination of Baicalin in Xiaoerqingfeihuatan Granule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(21): 112-114.
DOI：

TANG Hui-hui, CAI Qing-yu, ZHANG Li-juan. Determination of Baicalin in Xiaoerqingfeihuatan Granule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(21): 112-114. DOI：

摘要

目的: 建立高效液相色谱法测定小儿清肺化痰颗粒中黄芩苷的含量方法。方法: Agilent XDB C18色谱柱(4.6 mm×250 mm

4.6 μm);流动相:甲醇-0.4%磷酸溶液(47:53);流速1.0 mL ·min-1;检测波长280 nm柱温:40℃。结果: 回归方程为A=34 884.9C-19 478.8

R2=1.000 0。黄芩苷在1.482~148.2 mg ·L-1线性关系良好

平均回收率为97.8%

RSD 1.9%(n=6)。结论: 该方法简便、准确、重复性好

可作为小儿清肺化痰颗粒黄芩苷含量的测定。

Abstract

Objective: To establish a method for the determination of baicalin in Xiaoerqingfeihuatan granule by HPLC. Method: HPLC was used on an Agilent XDB C18 (4.6 mm×250 mm

4.6 μm) column with acetonitrile-0.4% phosphoric acid (47: 53) as mobile phase. The flow rate was 1.0 mL·min-1 with detection wavelength at 280 nm and column temperature at 40℃. Result: The regression equation of baicalin is A=34 884.9C-19 478.8

R2=1.000 0 (n=6). The linear range of baicalin was from 1.482-148.2 g ·L-1. The average recovery was 97.8%

and RSD was 1.9% (n=6). Conclusion: The method is simple

fast and accurate with good reproducibility. It can be used for the determination of Xiaoerqingfeihuatan granule.

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