Optimization of Extracting Technology for Total Alkaloids from by Enzymatic Hydrolysis Assisted Immersion Method

LU Shi-hui; DENG Feng-yun; LU Hong-mei; CHEN Nan; LIN Yao

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Optimization of Extracting Technology for Total Alkaloids from by Enzymatic Hydrolysis Assisted Immersion Method

更新时间：2024-01-04

- Optimization of Extracting Technology for Total Alkaloids from by Enzymatic Hydrolysis Assisted Immersion Method
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 23, Pages: 43-45(2012)
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- Published：2012
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LU Shi-hui, DENG Feng-yun, LU Hong-mei, et al. Optimization of Extracting Technology for Total Alkaloids from by Enzymatic Hydrolysis Assisted Immersion Method[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(23): 43-45.
DOI：

LU Shi-hui, DENG Feng-yun, LU Hong-mei, et al. Optimization of Extracting Technology for Total Alkaloids from by Enzymatic Hydrolysis Assisted Immersion Method[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(23): 43-45. DOI：

摘要

目的: 优选酶解法辅助浸渍提取两面针中总生物碱的工艺。方法: 以总生物碱提取率为指标

采用单因素试验考察酶的种类、加酶量、pH、酶解温度及时间对酶解工艺的影响;以总生物碱提取率为指标

采用正交试验考察提取次数、提取时间及乙醇体积分数对两面针总生物碱浸渍提取工艺的影响。结果: 酶解工艺条件为纤维素酶、果胶酶与底物质量比均为1∶250

4倍量pH 5缓冲液

室温(30 ℃)下酶解30 min;最佳浸渍提取工艺为以12

3倍量60%乙醇溶液(盐酸质量浓度5 g·L-1)室温下浸渍3次

每次2 h

总生物碱提取率85.22%。结论: 该优选的工艺简单高效、节能环保

为工业生产提供实验依据。

Abstract

Objective: To optimize extracting process of total alkaloids from Zanthoxylum nitidum by enzymatic hydrolysis assisted immersion. Method: With extraction rate of total alkaloids as index

effect of 5 factors on enzymatic technology was investigated by single factor test

including kind of enzyme

enzyme dosage

temperature and time;extraction times

extraction time and the concentration of ethanol were chosen as factors

effects of 3 factors on immersion technology of total alkaloids from Z. nitidum was optimized by orthogonal test. Result: Optimized enzymatic hydrolysis process was as following:cellulase-substrate and pectinase-substrate of 1∶250

4 times buffer with pH 5

enzyme pretreating for 30 min at ambient temperature (30 ℃);Optimum immersion extracting technology was following:extracted 3 times with 60% ethanol of 12

3 times amount(hydrochloric acid of 5 g·L-1 )

at ambient temperature

2 h each time. Extraction rate of total alkaloids of 85.22% by this process. Conclusion: This optimized process was simple

efficient

energy saving and environmentally friendly

and it could provide experimental base for industrial production.

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Related Institution

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