Identification and Determination of

SHAO Shuai; YAN Ming-ming; BI Sheng-nan; WAN Zhi-qiang; HUANG Yao-ling

您当前的位置：

首页 >

文章列表页 >

Identification and Determination of

更新时间：2024-01-04

- Identification and Determination of
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 24, Pages: 101-103(2012)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2012
- 稿件说明：
移动端阅览
SHAO Shuai, YAN Ming-ming, BI Sheng-nan, et al. Identification and Determination of [J]. Chinese journal of experimental traditional medical formulae, 2012, 18(24): 101-103.
DOI：

SHAO Shuai, YAN Ming-ming, BI Sheng-nan, et al. Identification and Determination of [J]. Chinese journal of experimental traditional medical formulae, 2012, 18(24): 101-103. DOI：

摘要

目的: 探讨小飞蓬药材定性、定量检测方法。方法: 采用TLC鉴别小飞蓬中的槲皮素

HPLC测定小飞蓬中槲皮素的含量。结果: TLC以正己烷-乙酸丁酯-甲酸(6∶5∶1) 为展开系统

可以清晰地检测槲皮素; HPLC采用日本岛津Shimadzu C18(4.6 mm×250 mm

5 μm)色谱柱

流动相-甲醇-0.4%磷酸(47∶53)

检测波长257 nm

流速1 mL·min-1

柱温30 ℃

槲皮素在0.504~1.134 μg呈良好线性关系

平均回收率分别为97.97%%

RSD分别为1.2 %。结论: 该法可作为小飞蓬药材的定性、定量检测方法。

Abstract

Objective:To discuss detection method of the qualitative and quantitative of Erigeron canawdensis. Method: TLC was used to determine quercetin of E. canawdensis. The content of quercetin was determined by HPLC. Result: n-Hexane-butyl acetate-formic (6∶5∶1) is used as TLC deployment system. It may clearly detect quercetin; Shimadzu C18 (4.6 mm×250 mm

5 μm) column was used in HPLC with mobile phase methanol-0.4% phosphoric acid (47∶53). The detection wavelength was set at 257 nm and the flow rate was 1 mL·min-1; the column temperature was at 30 ℃.The linear range of quercetin were respectively 0.504-1.134 μg

and their average recovery were respectively 97.97%

RSD were respectively 1.2%. Conclusion: This method is used as detection method of the qualitative and quantitative of E. canawdensis.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Study on Quality Standard for Weitong Tablets

Study on Quality Standard of Weichangshu Capsule

开胸顺气丸(大蜜丸)质量标准研究

五味子糖浆质量标准研究

Study on Quality Standers for Tongbao Xiaojia Mixture

Related Author

CHEN Wu-su1

CAI Ri-qiang1

SUN Hong2

CHEN Xin-fa2

XIE You-liang

ZENG Hui-fang

LI Jun-zhe

SU Zi-ren

Related Institution

黑龙江中医药大学

齐齐哈尔中医院

齐齐哈尔医学院

Liaoning University of Traditional Chinese Medicine

Xiyuan Hospital， China Academy of Chinese Medical Sciences

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰