Objective:To establish a method for the determination of paeonol in Eczema Nanoemulsion spray by HPLC. Method: Using HPLC technique

we detected the samples by the C18 ODS column of Phenomenex company under the wave length at 274 nm

the chromatographic conditions were phenomenex C18 -ODS column

mobile phase of methanol-water (55∶45)

flow rate of 1.0 mL·min-1 and detection wave-length at 274 nm. Result: The standard curve was linear over the range of 10.0-100.0 mg·L-1

with the correlation coefficient of 0.999 9.It is steady in 48 hour. The average recovery was 99.70% with RSD 1.12%(n=9). The theoretical plates are above 6 000

separating degree is greater than 1.5. Conclusion: The analytical method is simple

rapid

sensitive

accurate; stability and reproducibility are good

and the method could be used to determine content of paeonol in a Chinese medicine Eczema Nanoemulsion spray.