Effects of Fengshiqing on Expression of OPG/RANKL, TNF- and IL-17 in Fibroblast-like Synoviocytes

KONG Xiang-ying; WU Wen-bin; SU Xiao-hui; WU Zhen-yu; JIANG Quan; LIN Na

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Effects of Fengshiqing on Expression of OPG/RANKL, TNF- and IL-17 in Fibroblast-like Synoviocytes

更新时间：2024-01-04

- Effects of Fengshiqing on Expression of OPG/RANKL, TNF- and IL-17 in Fibroblast-like Synoviocytes
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 22, Pages: 287-290(2012)
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- Published：2012
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KONG Xiang-ying, WU Wen-bin, SU Xiao-hui, et al. Effects of Fengshiqing on Expression of OPG/RANKL, TNF- and IL-17 in Fibroblast-like Synoviocytes[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(22): 287-290.
DOI：

KONG Xiang-ying, WU Wen-bin, SU Xiao-hui, et al. Effects of Fengshiqing on Expression of OPG/RANKL, TNF- and IL-17 in Fibroblast-like Synoviocytes[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(22): 287-290. DOI：

摘要

目的: 观察风湿清 (FSQ)含药血清对类风湿关节炎患者成纤维样滑膜细胞 (RA-HFLS)增殖及骨保护素(OPG)、细胞核因子κB受体活化因子配基(RANKL)、肿瘤坏死因子α(TNF-α)及白介素-17 (IL-17)表达的影响

初步探讨FSQ治疗类风湿性关节炎 (RA) 的机制。方法: 不同体积分数的FSQ含药血清与RA-HFLS共孵育

采用3-(4

5-二甲基-2-基)-5(3-羧基甲基苯基)-2-(4-磺酸苯基)-2H-四氮唑(MTS) 比色法检测细胞增殖活性

ELISA 法检测培养液上清中OPG、IL-17及TNF-α浓度

Western blot法检测RA-HFLS中RANKL的蛋白表达。结果: 5%~20% FSQ含药血清可以浓度依赖性抑制RA-HFLS的增殖;RA-HFLS可分泌一定水平的OPG

FSQ含药血清可浓度依赖性促进OPG的分泌;同时FSQ含药血清可显著抑制RANKL的表达;FSQ含药血清还可显著抑制由炎症因子诱导的RA-HFLS中IL-17及TNF-α的分泌

并呈现一定的浓度依赖性。结论: FSQ能显著抑制RA-HFLS的增殖

促进OPG分泌

并抑制RANKL

IL-17和TNF-α的水平

这可能是FSQ抑制骨破坏治疗RA的部分作用机制。

Abstract

Objective:To observe the effect of Fengshiqing (FSQ) conlaining serum on the expression of osteoprotegerin(OPG)

receptor activator for nuclear factor-κB ligand(RANKL)

tumor necrosis factor-α(TNF-α) and interleukin-17 (IL-17) in human fibroblast-like synoviocytes of rheumatoid arthritis(RA-HFLS). Method: RA-HFLS were cultured with different volume ration of FSQ in serum. Cell proliferation was evaluated by MTS assay.The expression of OPG

IL-17 and TNF-α in cell culture supernatant was examined by ELISA

and expression of RANKL was detected by Western blot analysis. Result: FSQ containing serum could obviously decrease the RA-HFLS viability in a dose-dependent style. The RA-HFLS could secret certain level of OPG

and FSQ containing surum could promote the secretion of OPG with a dose-dependent style. FSQ containing surum also suppressed the expression of RANKL

IL-17 and TNF-α. Conclusion: FSQ has an notable inhibiting effect on proliferation of RA-HFLS

increasing the level of OPG

and suppressing expression of RANKL

IL-17 and TNF-α

which may be a part of the mechanism of FSQ for rheumatoid arthritis.

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Related Institution

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