Effects and Mechanism of Supplemental Sini San on Hepatic Fibrosis in Rats

SHANG Li-zhi; WANG Fu; MIAO Xiao-ling; ZHANG Hui-na; GAN Chen-fei

您当前的位置：

首页 >

文章列表页 >

Effects and Mechanism of Supplemental Sini San on Hepatic Fibrosis in Rats

更新时间：2024-09-23

- Effects and Mechanism of Supplemental Sini San on Hepatic Fibrosis in Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 18, Pages: 194-198(2012)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2012
- 稿件说明：
移动端阅览
SHANG Li-zhi, WANG Fu, MIAO Xiao-ling, et al. Effects and Mechanism of Supplemental Sini San on Hepatic Fibrosis in Rats[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 194-198.
DOI：

SHANG Li-zhi, WANG Fu, MIAO Xiao-ling, et al. Effects and Mechanism of Supplemental Sini San on Hepatic Fibrosis in Rats[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 194-198. DOI：

摘要

目的: 探讨四逆散加味对肝纤维化的防治作用机制。方法: 80只Wister大鼠随机分为8组:正常对照组、病理模型组、四逆散组、四逆散加味高、中、低剂量组、四逆散预防组。除正常对照组外

其余各组均采用猪血清ip诱发肝纤维化

0.5 mL/只

2次/周

连续10周

5周后即可形成肝纤维化。预防组于造模同时给药(以四逆散加味7 g ·kg-1)

各治疗组于造模第6周给药

连续4周。四逆散组4 g ·kg-1

四逆散加味高、中、低剂量组(14

3.5 g ·kg-1)。采用酸性水解法检测肝组织羟脯氨酸(HYP)含量;放射免疫法(RIA)检测肝组织白介素-1(IL-1)和肿瘤坏死因子-α(TNF-α)含量;原位杂交、免疫组化法分别检测肝组织转化生长因子-β1 (TGF-β1)和α-平滑肌肌动蛋白(α-SMA)mRNA与蛋白表达。结果: 与模型组比较

四逆散加味中剂量组大鼠肝组织羟脯氨酸(478.32±42.35) vs (327.09±39.41) μg ·L-1

P<0.01; IL-1(0.58±0.89) vs (0.35±0.47) μg ·L-1(P<0.05); TNF-α(4.82±0.49) vs (4.21±0.45) μg ·L-1(P<0.05)

含量均显著降低

TGF-β1 mRNA(9.92±2.57) vs (5.27±1.39)

(P<0.01)和α-SMA mRNA(12.87±0.39) vs (6.33±0.72)

(P<0.01)及其蛋白表达显著减弱(均P<0.01)。结论: 四逆散加味有明显的抗肝纤维化作用

其机制可能与抑制TGF-β1和α-SMA基因表达有关。

Abstract

Objective: To investigate the protective effects and its mechanism of Supplemental Sini San(SNS) on hepatic fibrosis in rats. Method: The immunohepatic fibrosis model was induced by intraperitoneal injection of porcine serum. Eighty Wister rats were divided into eight groups:normal control

model control

Sini San Jiawei(SNSJW) high-dose

mediate-dose and low-dose

prevention and SNS group. Except the normal control groups

all the other groups were injected with pig's serum without inactivation into abdominal cavity

twice per week and 0.5 mL each time

persisting for 10 weeks. The prevention groups were orally given with the medicines(SNSJW 7 g · kg-1

10 weeks)at the same time models wasduplicated

the other treatment groups were dealed with just as the prevention groups 6 weeks later

lasted for 4 weeks. The dose was following:SNS group 4 g · kg-1

SNSJW high-dose group 14 g · kg-1

mediate-dose group 7 g · kg-1

low-dose group 3.5 g · kg-1. The changes of hepatic fibrosis were detected. The content of liver tissue interleukin-1(IL-1) and tumor necrosis factor-alpha (TNF-α)were tested by RIA. In situ hybridization assay and immunohistochemical S-P method were used to detect the expression of transforming growth factor-β1(TGF-β1) mRNA and α-smoth muscle actin(α-SMA) mRNA and there protein in hepatic fibrosis tissues. Result: Compared with model group in SNSJW mediate-dose group

the content of HYP

IL-1

TNF-α in liver tissue was depressed significantly

the expression of TGF-β1 and α-SMA genes was significantly lower in SNSJW mediate-dose group. Conclusion: Supplemental SNS has an action on hepatic fibrosis in rats. The mechanism is related with its inhibiting the expression of TGF-β1 and α-SMA genes in rats liver.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effects and Mechanism of Supplemental Sini San on Hepatic Fibrosis in Rats

Effects of Supplemental Sini San on Hepatic Function and Liver Fibrosisin in Rat Hepatic Fibrosis Model

Observation of 42 Cases of Therapeutic Effects of Liuwei Wuling Tablet in Treatment of Hepatitis B with Hepatic Fibrosis

Effect of Kangxian'gan Decoction in Treating 54 Cases with Liver Fibrosis Caused by Chronic Hepatitis B

Effect of Plumbagin on -SMA Expressions in Human Hepatic Stellate Cells Stimulated by TGF-

Related Author

SHANG Li-zhi

WANG Fu

MIAO Xiao-ling

ZHANG Hui-na

GAN Chen-fei

WANG Fu

SHANG Li-zhi

LI Guang-ming

Related Institution

Department of Physiology,Henan University of Traditional Chinese Medicine

Henan General Hospital

The First Affiliated Hospital of Henan University of Chinese Medicine

The First Clinical Medical College of Henan University of Chinese Medicine

The Healthcare Center for Shishan Street of Suzhou New District

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰