Optimization of Enrichment and Purification Technology for Total Glycosides fromby Macroporous Resin
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Optimization of Enrichment and Purification Technology for Total Glycosides fromby Macroporous Resin
Chinese Journal of Experimental Traditional Medical FormulaeVol. 18, Issue 19, Pages: 24-28(2012)
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Published:2012
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LI Ying, LI Yan-dan, LIU Yuan, et al. Optimization of Enrichment and Purification Technology for Total Glycosides fromby Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(19): 24-28.
DOI:
LI Ying, LI Yan-dan, LIU Yuan, et al. Optimization of Enrichment and Purification Technology for Total Glycosides fromby Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(19): 24-28.DOI:
Optimization of Enrichment and Purification Technology for Total Glycosides fromby Macroporous Resin
Objective:To optimize technology conditions and technical parameters of enrichment and purification process for total glycosides from Acanthopanax giraldii by macroporous resin. Method:A RP-HPLC method was adopted to determine the content of total glycosides in A. giraldii with elution ratio of syringin and oleanolic acid as indexes
adsorption characteristics and elution parameters of enrichment and purification technology were investigated from 10 kinds of macroporous resin. Result:X-5 was the best macroporous resin;Optimum purification technology was:the concentration of total glycosides extraction from A. giraldii 0.3 g·mL-1
pH of liquid 10
eluted with 4 BV 80% ethanol. Conclusion:X-5 macroporous resin could be well used for enrichment and purification of total glycosides from A. giraldii.
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Investigation of Adsorption and Desorption Properties of Total Ginsenosides on Anion-exchange Resins and Its LC-MS Analysis
LCMS-IT-TOF Analysis of Retention Behavior of Sanguisorbae Radix Components on D101 Macroporous Resin and Polyamide Resin
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