Effects of Extract of Corni Frutus on Tyrosinase, Tyrosinase Related Protein-1 Tyrosinase Related Protein-2 Expression of Melanoma Cells and Keratinocytes in Co-culture Model
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Effects of Extract of Corni Frutus on Tyrosinase, Tyrosinase Related Protein-1 Tyrosinase Related Protein-2 Expression of Melanoma Cells and Keratinocytes in Co-culture Model
Chinese Journal of Experimental Traditional Medical FormulaeVol. 18, Issue 22, Pages: 283-286(2012)
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Published:2012
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LI Shao-min, WANG Xing-yan, ZHANG Ning, et al. Effects of Extract of Corni Frutus on Tyrosinase, Tyrosinase Related Protein-1 Tyrosinase Related Protein-2 Expression of Melanoma Cells and Keratinocytes in Co-culture Model[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(22): 283-286.
DOI:
LI Shao-min, WANG Xing-yan, ZHANG Ning, et al. Effects of Extract of Corni Frutus on Tyrosinase, Tyrosinase Related Protein-1 Tyrosinase Related Protein-2 Expression of Melanoma Cells and Keratinocytes in Co-culture Model[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(22): 283-286.DOI:
Effects of Extract of Corni Frutus on Tyrosinase, Tyrosinase Related Protein-1 Tyrosinase Related Protein-2 Expression of Melanoma Cells and Keratinocytes in Co-culture Model
Objective:To observe the effects of the extract of Corni Fructus on the mRNA expression of tyrosinase(TYR)
tyrosinase related protein-1(TRP-1) and TRP-2 in co-culture model of melanoma cells and keratinocytes. Method: Differences concentration of the extract of Corni Fructus(1.0
0.5 g·L-1) were added in co-culture model of A375 melanoma cells and HaCat keratinocytes.RT-PCR assay was used to detect the effects of the extract of Corni Fructus on the TYR
TRP-1 and TRP-2 mRNA expression in A375 melanoma cells. Result: Compared with control group
the extract of Corni Fructus(1.0
0.5 g·L-1) down-regulated mRNA expression of TYR
TRP-1 and TRP-2 in A375 melanoma cells by 36.07%
62.34%
44.69% and 24.10%
53.00%
16.11% respectively(P<0.01). Conclusion: The extract of Corni Fructus can decrease the melanogenesis by down-regulating the mRNA expression of tyrosinase and its related protein.
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