Proliferation and Apoptosis Effects of Gypenosides on Human Hepatocellular Carcinoma Bel-7402 Cells

LI Xiao-long; WU Yu-lang; LIN Zi-qun; MO Miao; LIANG Ya-xing; PAN Yue; LIU Shi-liang

您当前的位置：

首页 >

文章列表页 >

Proliferation and Apoptosis Effects of Gypenosides on Human Hepatocellular Carcinoma Bel-7402 Cells

更新时间：2024-01-04

- Proliferation and Apoptosis Effects of Gypenosides on Human Hepatocellular Carcinoma Bel-7402 Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 19, Pages: 238-241(2012)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2012
- 稿件说明：
移动端阅览
LI Xiao-long, WU Yu-lang, LIN Zi-qun, et al. Proliferation and Apoptosis Effects of Gypenosides on Human Hepatocellular Carcinoma Bel-7402 Cells[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(19): 238-241.
DOI：

LI Xiao-long, WU Yu-lang, LIN Zi-qun, et al. Proliferation and Apoptosis Effects of Gypenosides on Human Hepatocellular Carcinoma Bel-7402 Cells[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(19): 238-241. DOI：

摘要

目的:探讨绞股蓝总皂苷(gypenosides

Gyp)对人肝癌细胞Bel-7402增殖与凋亡的影响。方法:采用MTT法检测Gyp对体外培养的人肝癌细胞Bel-7402的增殖抑制作用;单细胞凝胶电泳法检测Gyp对人肝癌细胞Bel-7402DNA损伤的影响;RT-PCR技术从分子水平上检测Gyp对人肝癌细胞Bel-7402的诱导凋亡作用机制。结果:100~400μg.mL-1的Gyp对人肝癌细胞Bel-7402增殖生长有抑制作用

Gyp(182μg·mL-1)作用4

24h后

单细胞凝胶电泳观测到DNA损伤

12h时DNA损伤最大

达到(81.15±14.23)%

且有时间依赖性;Gyp组细胞caspase-8大量表达

对照组细胞无表达。结论:Gyp能抑制人肝癌细胞Bel-7402增殖且促进其凋亡

其作用机制与DNA损伤以及caspase-8的大量表达有关。

Abstract

Objective:To investigate the suppressive effect of gypenosides(Gyp) on human hepatocellular carcinoma Bel-7402 cells. Method:The growth inhibitation of human hepatocellular carcinoma Bel-7402 cells was detected by MTT assay. The DNA damage of human hepatocellular carcinoma Bel-7402 cells was detected by single cell gel electrophoresis; RT-PCR was utilized to investigate the apoptosis of human hepatocellular carcinoma Bel-7402 cells and its relationship with caspase-8 from molecule level. Result:Gyp (100-400 μg·mL-1) inhibied the growth of human hepatocellular carcinoma Bel-7402 cells. DNA damage was detected after Gyp (182 μg·mL-1) treated for 4

24 h. The change reached maximum at 12 h(81.15±14.23)% and with time-dependent. caspase-8 expressed strongly in Gyp group and nothing in control. Conclusion:Gyp has effects on cell viability

it could induce human hepatocellular carcinoma Bel-7402 cells apoptosis

DNA damage and caspase-8 expression.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Inhibition of IGF-1R, Akt and Bcl-2 by Curcumae Rhizoma Oil on Human Osteosarcoma saos-2 Cells

Related Author

ZHU Fu-liang

LIU Jin-yang

HUANG Feng-xiang

Related Institution

No data

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰