Influence of Sheng-huang Mixtrue on Apoptosis Related Proteins Bax, Bcl-2 and Caspase-3 in Myocardial Ischemia-reperfusion Rats

GAO Yu-feng; WANG Xiao-jie; YAN Wen-cui; ZHANG Wei-na; LI Xin; ZHANG Feng-ying

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Influence of Sheng-huang Mixtrue on Apoptosis Related Proteins Bax, Bcl-2 and Caspase-3 in Myocardial Ischemia-reperfusion Rats

更新时间：2024-01-04

- Influence of Sheng-huang Mixtrue on Apoptosis Related Proteins Bax, Bcl-2 and Caspase-3 in Myocardial Ischemia-reperfusion Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 13, Pages: 244-247(2012)
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- Published：2012
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GAO Yu-feng, WANG Xiao-jie, YAN Wen-cui, et al. Influence of Sheng-huang Mixtrue on Apoptosis Related Proteins Bax, Bcl-2 and Caspase-3 in Myocardial Ischemia-reperfusion Rats[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(13): 244-247.
DOI：

GAO Yu-feng, WANG Xiao-jie, YAN Wen-cui, et al. Influence of Sheng-huang Mixtrue on Apoptosis Related Proteins Bax, Bcl-2 and Caspase-3 in Myocardial Ischemia-reperfusion Rats[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(13): 244-247. DOI：

摘要

目的: 观察生黄合剂对缺血再灌注损伤大鼠心肌细胞凋亡相关基因Bcl-2 相关X蛋白(Bax)、B型白细胞/2型淋巴细胞样蛋白(Bcl-2)和天冬氨酸特异性半胱氨酸蛋白酶(Caspase-3)蛋白表达的影响。方法: 将48只SD大鼠随机分为6组

即对照组、模型组、阳性药物组、生黄合剂低、中、高剂量组(17.5

70 mg·kg-1分别加入5 mL·kg-1生脉饮)

每组8只

分别给药7 d后

建立心肌缺血再灌注损伤(MIRI)模型。采用原位末端标记法(TUNEL)检测心肌细胞凋亡情况

蛋白印迹法检测心肌细胞凋亡相关基因Bax

Bcl-2

Caspase-3蛋白的表达。结果: 与模型组比较

生黄合剂能减少心肌细胞凋亡指数(AI)及Bax

Caspase-3蛋白的表达

增加Bcl-2蛋白的表达

提高Bcl-2/Bax的比值(P<0.05)

其中以生黄合剂高剂量组作用较为明显。结论: 生黄合剂对大鼠心肌缺血再灌注损伤具有保护作用

其抗心肌细胞凋亡的机制可能与通过上调Bcl-2

下调Bax和Caspase-3基因表达

提高Bcl-2/Bax的比值有关。

Abstract

Objective: To investigate the influence of Sheng-huang mixtrue on the apoptosis related proteins expression of Bcl-assosiated x protein(Bax)

B-cell leukemia/lymphoma 2-like proteins(Bcl-2) and Aspartate-specific cysteinyl proteinase(Caspase-3) in myocardial ischemia-reperfusion injury rats. Method: SD rats(n=48) were divided randomly into six groups: normal control group

model group

positive control group

low-dose

medium-dose and high-dose Sheng-huang mixtrue groups(n=8). After the drug was administered to the six groups seven days

the Myocardial Ischemia-reperfusion injury in rats was established. TUNEL was used to detect the myocardial apoptosis index

The protein expressions of Bax

Bcl-2 and Caspase-3 were assayed by western blotting. Result: In comparison of the five other groups with model group

Sheng-huang mixture can decrease the myocardial apoptosis index and the protein expressions of Bax and Caspase-3

but increase the protein expressions of Bcl-2 in rats and the ratio of Bcl-2/Bax. Among which high dose Sheng-huang mixtrue group decreased much more than other groups. Conclusion: Sheng-huang mixture has the protective effects on myocardial ischemia-reperfusion injury in rats

which can inhibit the myocardial apoptosis of myocardial ischemia-reperfusion injury by increasing the protein expressions of Bcl-2 and reducing the protein expressions of Bax and Caspase-3.

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