HPLC Simultaneous Determination of 7 Components in Shuanghuangqudu Tablets

CHEN Yun-long; LU Xiao-feng; LIAO Jin-bin; LIN Rong-feng; HUANG Xiao-qi; YI Yu-yang; SU Zi-ren

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HPLC Simultaneous Determination of 7 Components in Shuanghuangqudu Tablets

更新时间：2024-01-04

- HPLC Simultaneous Determination of 7 Components in Shuanghuangqudu Tablets
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 13, Pages: 119-122(2012)
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- Published：2012
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CHEN Yun-long, LU Xiao-feng, LIAO Jin-bin, et al. HPLC Simultaneous Determination of 7 Components in Shuanghuangqudu Tablets[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(13): 119-122.
DOI：

CHEN Yun-long, LU Xiao-feng, LIAO Jin-bin, et al. HPLC Simultaneous Determination of 7 Components in Shuanghuangqudu Tablets[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(13): 119-122. DOI：

摘要

目的: 建立测定双黄祛毒片(盐酸小檗碱、大黄、五倍子)含量的方法。方法: 采用HPLC在同一色谱条件ZORBAX SB-Pheny 色谱柱(4.6 mm×150 mm

5 μm)对没食子酸、盐酸小檗碱、大黄酸、大黄酚、芦荟大黄素、大黄素、大黄素甲醚进行含量测定。结果: 线性范围分别为0.12~2.4 μg(r=0.999 9)

盐酸小檗碱线性范围0.11~2.2 μg(r=0.9999)

0.015~0.3 μg(r=0.9998)

0.02~0.4 μg(r=0.9998)

0.008~0.16 μg(r=0.9999)

0.013~0.26 μg(r=0.9998)

0.006~0.12 μg(r=0.9999)

平均回收率分别为100.97%

100.20%

99.01%

99.76%

101.10%

101.97%

101.56%。结论: 该方法简便、快速、准确

重复性好

可作为双黄祛毒片中没食子酸、盐酸小檗碱、大黄酸、大黄酚、芦荟大黄素、大黄素、大黄素甲醚含量测定方法。

Abstract

Objective: To establish a method for the determination of gallic acid

berberine hydrochloride

aloe emodin

rhein

emodin

chrysophanol and physcion in Shuanghuangqudu tablets. Method: The HPLC system consisted of a ZORBAX SB-Pheny Dimensions column(4.6 mm×15 cm

5 μm)

methanol-0.1% phosphoric acid as mobile phase (using gradient) with flowing rate 1.0 mL·min-1 and detected at 310 nm. Result: The linear ranges of gallic acid

berberine hydrochloride

aloe emodin

rhein

emodin

chrysophanol and physcion were 0.12-2.4 μg (r=0.9999)

0.11-2.2 μg (r=0.9999)

0.015-0.3 μg (r=0.9998)

0.02-0.4 μg (r=0.9998)

0.008-0.16 μg (r=0.9999)

0.013-0.26 μg (r=0.9998)

0.006-0.12 μg (r=0.9999)

respectively

and the average recoveries (n=9) were 100.97%

100.20%

99.01%

99.76%

101.10%

101.97%

100.56%

respectively. Conclusion: The method is simple and repeatable

which can be applied to determine seven ingriedients at the same test condition in Shuanghuangqudu tablets.

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Related Institution

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