HPLC Determination of Naringin, Hesperidin and Neohesperidin in Citri Reticulatae Pericarpium and Aurantii Fructus Extract

GUO Qi-li; LU Wu-qing; ZENG Li-ping; LI Cai-tang

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HPLC Determination of Naringin, Hesperidin and Neohesperidin in Citri Reticulatae Pericarpium and Aurantii Fructus Extract

更新时间：2024-01-04

- HPLC Determination of Naringin, Hesperidin and Neohesperidin in Citri Reticulatae Pericarpium and Aurantii Fructus Extract
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 20, Pages: 100-103(2012)
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- Published：2012
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GUO Qi-li, LU Wu-qing, ZENG Li-ping, et al. HPLC Determination of Naringin, Hesperidin and Neohesperidin in Citri Reticulatae Pericarpium and Aurantii Fructus Extract[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(20): 100-103.
DOI：

GUO Qi-li, LU Wu-qing, ZENG Li-ping, et al. HPLC Determination of Naringin, Hesperidin and Neohesperidin in Citri Reticulatae Pericarpium and Aurantii Fructus Extract[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(20): 100-103. DOI：

摘要

目的: 建立陈皮-枳壳药对提取物中柚皮苷、橙皮苷和新橙皮苷的含量测定方法。方法: 采用HPLC

Agilent Hypersill C18(4.0 mm×250 mm

5 μm)色谱柱

以乙腈-0.1%磷酸(24∶76)为流动相

流速1 mL·min-1

检测波长283 nm

柱温40 ℃。结果: 柚皮苷、橙皮苷和新橙皮分别在6.05~60.5

4.34~43.4

7.14~71.40 mg·L-1线性关系良好(r均>0.999 5);加样回收率分别为98.65%

98.66%

100.78%

其RSD分别为2.01%

1.52%

2.11%;重复性RSD分别为2.41%

1.56%

2.52% (n=6)。结论: 该方法简便可行

重复性好

结果准确可靠

可用于陈皮-枳壳药对提取物中柚皮苷、橙皮苷和新橙皮苷的含量测定。

Abstract

Objective:To develop a method to determine naringin

hesperidin and neohesperidin in Citri Reticulatae Pericarpium and Aurantii Fructus. Method: HPLC was used to determine directly naringin

hesperidin and neohesperidin of Citri Reticulatae Pericarpium and Aurantii Fructus. Phenomsil C18(4.0 mm×250 mm

5 μm)as the chromatographic column

and acetonitrile-10% aqueous phosphoric acid (24∶76)as the mobile phase

the flow rate of 1.0 mL·min-1

the column temperature was at 40 ℃. Result: The linearity of verbascoside was good in the range of 6.05-60.5

4.34-43.4 and 7.14-71.40 mg·L-1(r>0.999 5).The recovery of the added sample was 98.65%

98.66% and 100.78% with RSD of 2.01%

1.52% and 2.11%. Conclusion: The method is simple

accurate and can be used for the determination of naringin

hesperidin and neohesperidin in Tangerine peel and Aurantii Fructus extract.

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