Objective: The anti-fatigue and immune properties by nutritional constituents from Synsepalum dulcificum mixing averrhoa carambola

carica papaya and pumpkin fruit powders (SDMFP) were evaluated. Method: The nutrition ingredients from SDMFP were analyzed as follows

the amount of total sugar using Fehling reagent hot titration

the content of moisture by directly dried

contents of crude fat and protein

were assayed by acid hydrolysis and Kieldahl method respectively

trace elements contents were determined through the atomic absorption method. The male mice were divided randomly into 4 groups: blank control group

low

medium

high group(SDMFP

100

200

400 mg·kg-1·d-1). The SDMFP were orally given to mice daily for 14 days

the loaded swimming time and some biochemistry indexes including blood lactic acid (BLA)

hepatic glycogen (HG)

and blood urea nitrogen (BUN) were tested to evaluate the anti-fatigue property. The function of macrophage cell and the delayed type hypersensitivity (DTH) were detected for the immune function. Result: The analysis result of SDMFP was: total sugar 42.12%

crude fat 1.97%

protein 0.33%

water 11.31%

and the content of K

Mg

Ca were 5 926.15

826.75

600.83 μg·g-1 respectively. Compared with the blank group

the mice in the high group (SDMFP

400 mg·kg-1·d-1) prolonged the loaded swimming time (P<0.01)

reduced the blood lactic acid and blood urea nitrogen

and the content of hepatic glycogen were increased (P<0.05)

the index of phagocytic macrophage cell and the weight of thymus and spleen were increased highly. Conclusion: The research results showed that the SDMFP could significantly enhance the ability of anti-fatigue and heighten the immune function of mice at a certain extent.