HPLC-ELSD Determination of Content of Oleanane-type Triterpenoidal Saponins in the Fruits of

ZHANG Zheng-guang; ZHU Ling-ying; QIAN Shi-hui

您当前的位置：

首页 >

文章列表页 >

HPLC-ELSD Determination of Content of Oleanane-type Triterpenoidal Saponins in the Fruits of

更新时间：2024-09-23

- HPLC-ELSD Determination of Content of Oleanane-type Triterpenoidal Saponins in the Fruits of
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 18, Pages: 81-84(2012)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2012
- 稿件说明：
移动端阅览
ZHANG Zheng-guang, ZHU Ling-ying, QIAN Shi-hui. HPLC-ELSD Determination of Content of Oleanane-type Triterpenoidal Saponins in the Fruits of [J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 81-84.
DOI：

ZHANG Zheng-guang, ZHU Ling-ying, QIAN Shi-hui. HPLC-ELSD Determination of Content of Oleanane-type Triterpenoidal Saponins in the Fruits of [J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 81-84. DOI：

摘要

目的: 建立HPLC-ELSD法同时测定细柱五加果实中的竹节参苷Ⅳa和3β-([O-β-D-glucopyranuronosyl]oxy)-olean-12-ene-28-olc acid含量的方法。方法: 采用反相高效液相色谱-蒸发光散射检测法。色谱条件AlltimaTM-C18色谱柱(4.6 mm×250 mm

5 μm)

流动相乙腈(A)-0.5%乙酸水溶液(B)

柱温35℃。ELSD检测器条件:漂移管温度106℃

载气氮气

流速2.9 L · min-1

放大系数1

撞击器关闭。结果: 竹节参苷Ⅳa和3β-( oxy)-olean-12-ene-28-olc acid的进样量分别在0.535~8.560 μg (r=0.996 5)和0.515~8.240 μg (r=0.9987)呈良好线性关系;平均回收率(n=6)分别为98.39%

98.97%

RSD分别为1.50%

1.59%。结论: 方法准确、灵敏

可用于测定细柱五加果实中竹节参苷Ⅳa和3β-( oxy)-olean-12-ene-28-olc acid的含量。

Abstract

Objective: To establish an HPLC-ELSD method for the simultaneous determination of the content of chikusetsusaponin Ⅳa and 3β -([[BFQ]O-β-D-glucopyranuronosyl]oxy)-olean-12-ene-28-olc acid in the fruits of Acanthopanax gracilistylus. Method: The RP-HPLC-ELSD method was used. Chromatographic conditions:AlltimaTM-C18 chromatographic column (4.6 mm×250 mm

5 μm);acetonitrile(A)-0.5% acetic acid water(B) as mobile phase;column temperature at 35℃. ELSD detector conditions:drift tube temperature: 106℃;carrier gas(nitrogen) flow rate: 2.9 L · min-1;amplification factor:1;impactor:close. Result: The calibration curves showed good linear relationship in the range from 0.535 to 8.560 μg (r=0.996 5) for chikusetsusaponin Ⅳa and 0.515 to 8.240 μg (r=0.998 7) for 3β-( oxy)-olean-12-ene-28-olc acid. The average recovery rates(n=6) were 98.39% and 98.97%

and RSDs were 1.50% and 1.59%. Conclusion: This method is accurate

sensitive and can be used to determine the content of chikusetsusaponin Ⅳa and 3β-( oxy)-olean-12-ene-28-olc acid in the fruits of Acanthopanax gracilistylus.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

HPLC-ELSD Determination of Content of Oleanane-type Triterpenoidal Saponins in the Fruits of Acanthopanax gracilistylus

The Immunoregulation of Extracts from Fruits of in Immunosuppressive Mice

Comparative Study on Content of Medicinal Materials from Three Varieties of Sambucus williamsii

Separation,Purification and Determination of Protein in

Determination of Sipeimine in Shedan Chuanbei Powder by HPLC-ELSD

Related Author

QIAN Shi-hui1

ZHU Ling-ying1

ZHANG Zheng-guang1

HUANG Hou-cai

Jun-xiu YAO

Dan-dan SUN

De-jun WU

Qing-mei GUO

Related Institution

Affiliated Hospital of Nanjing University of Chinese Medicine on Intergrationof Chinese and Western Medicine

Jiangsu Provincial Insititute of Traditional Chinese Medicine

Shandong University of Traditional Chinese Medicine

Shandong Academy of Forestry

Pingyin County Forestry Bureau

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰